How do in vitro reconstituted actin-based motility assays provide insight into in vivo behavior?

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  • Author(s): Bugyi B;Bugyi B; Le Clainche C; Romet-Lemonne G; Carlier MF
  • Source:
    FEBS letters [FEBS Lett] 2008 Jun 18; Vol. 582 (14), pp. 2086-92. Date of Electronic Publication: 2008 Mar 05.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't; Review
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: John Wiley & Sons Ltd Country of Publication: England NLM ID: 0155157 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0014-5793 (Print) Linking ISSN: 00145793 NLM ISO Abbreviation: FEBS Lett Subsets: MEDLINE
    • Publication Information:
      Publication: Jan. 2016- : West Sussex : John Wiley & Sons Ltd.
      Original Publication: Amsterdam, North-Holland on behalf of the Federation of European Biochemical Societies.
    • Subject Terms:
      Biological Assay* ; Cell Movement*; Actins/*metabolism ; Pseudopodia/*physiology; Actin Capping Proteins/metabolism ; Actin-Related Protein 2-3 Complex/metabolism ; Destrin/metabolism
    • Abstract:
      Recent live cell image analysis of actin dynamics in lamellipodia of motile cells has shown that regulated treadmilling, which supports actin-based propulsion of functionalized particles in biomimetic reconstituted motility assays, is also responsible for lamellipodia extension. In both cases, filaments are created by branching with Arp2/3 complex only at the membrane or particle surface, grow transiently and are capped; ADF/cofilin enhances the treadmilling but does not sever filaments in the body of the meshwork. Differences between the cellular and biomimetic systems suggest that additional regulatory mechanisms take place in lamellipodia.
    • Number of References:
      66
    • Accession Number:
      0 (Actin Capping Proteins)
      0 (Actin-Related Protein 2-3 Complex)
      0 (Actins)
      0 (Destrin)
    • Publication Date:
      Date Created: 20080311 Date Completed: 20080807 Latest Revision: 20080603
    • Publication Date:
      20231215
    • Accession Number:
      10.1016/j.febslet.2008.02.065
    • Accession Number:
      18328266