Expression profile identifies novel genes involved in neuronal differentiation.

Publisher: Korean Society for Biochemistry and Molecular Biology Country of Publication: Korea (South) NLM ID: 101465334 Publication Model: Print Cited Medium: Print ISSN: 1976-6696 (Print) Linking ISSN: 19766696 NLM ISO Abbreviation: BMB Rep Subsets: MEDLINE

Original Publication: Seoul : Korean Society for Biochemistry and Molecular Biology

Gene Expression Profiling* ; Oligonucleotide Array Sequence Analysis*; Biomarkers/*metabolism ; Cell Differentiation/*genetics ; Neurons/*cytology; Animals ; Blotting, Western ; Cells, Cultured ; Gene Expression Regulation ; Heme Oxygenase (Decyclizing)/antagonists & inhibitors ; Heme Oxygenase (Decyclizing)/genetics ; Heme Oxygenase (Decyclizing)/metabolism ; Nerve Growth Factor/pharmacology ; Neurons/metabolism ; PC12 Cells ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; RNA, Small Interfering/pharmacology ; Rats ; Reverse Transcriptase Polymerase Chain Reaction ; Transfection

In the presence of NGF, PC12 cells extend neuronal processes, cease cell division, become electrically excitable, and undergo several biochemical changes that are detectable in developing sympathetic neurons. We investigated the expression pattern of the apoptosis-related genes at each stage of neuronal differentiation using a cDNA microarray containing 320 apoptosis-related rat genes. By comparing the expression patterns through time-series analysis, we identified candidate genes that appear to regulate neuronal differentiation. Among the candidate genes, HO2 was selected by real-time PCR and Western blot analysis. To identify the roles of selected genes in the stages of neuronal differentiation, transfection of HO2 siRNA in PC12 cells was performed. Down-regulation of HO2 expression causes a reduction in neuronal differentiation in PC12 cells. Our results suggest that the HO2 gene could be related to the regulation of neuronal differentiation levels.

0 (Biomarkers)
0 (RNA, Messenger)
0 (RNA, Small Interfering)
9061-61-4 (Nerve Growth Factor)
EC 1.14.14.18 (Heme Oxygenase (Decyclizing))
EC 1.14.14.18 (heme oxygenase-2)

Date Created: 20080305 Date Completed: 20080519 Latest Revision: 20190608

20221213

10.5483/bmbrep.2008.41.2.146

18315951