Molecular design of photosynthesis-elevated chloroplasts for mass accumulation of a foreign protein.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Oxford University Press Country of Publication: Japan NLM ID: 9430925 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1471-9053 (Electronic) Linking ISSN: 00320781 NLM ISO Abbreviation: Plant Cell Physiol Subsets: MEDLINE
    • Publication Information:
      Publication: Tokyo : Oxford University Press
      Original Publication: Kyoto, Japan : Japanese Society of Plant Physiologists,
    • Subject Terms:
      Chloroplasts/*metabolism ; Fructose-Bisphosphatase/*metabolism ; Genetic Engineering/*methods ; Phosphoric Monoester Hydrolases/*metabolism ; Photosynthesis/*physiology ; Nicotiana/*genetics; Fructose-Bisphosphatase/genetics ; Gene Expression Regulation, Plant ; Phosphoric Monoester Hydrolases/genetics ; Plant Leaves/enzymology ; Plants, Genetically Modified ; Nicotiana/enzymology
    • Abstract:
      In order to increase production of a useful protein by the chloroplast transformation technique, it seems to be necessary to determine the upper limit for the accumulation of a biologically active foreign protein in chloroplasts and then improve photosynthetic capacity and plant productivity. Here we show that the stromal fractions of tobacco chloroplasts could accommodate an additional 200-260 mg ml(-1) of green fluorescent protein in the stroma without any inhibition of gas exchange under various light intensity and growth conditions. The minimum amount of fructose-1,6-/sedoheptulose-1,7-bisphosphatase (FBP/SBPase) limiting photosynthesis was then calculated. Analyses of the photosynthetic parameters and the metabolites of transformants into which FBP/SBPase was introduced with various types of promoter (PpsbA, Prrn, Prps2 and Prps12) indicated that a 2- to 3-fold increase in levels of FBPase and SBPase activity is sufficient to increase the final amount of dry matter by up to 1.8-fold relative to the wild-type plants. Their increases were equivalent to an increase of <1 mg ml(-1) of the FBP/SBPase protein in chloroplasts and were calculated to represent <1% of the protein accumulated via chloroplast transformation. Consequently, >99% of the additional 200-260 mg ml(-1) of protein expressed in the chloroplasts could be used for the production of useful proteins in the photosynthesis-elevated transplastomic plants having FBP/SBPase.
    • Accession Number:
      EC 3.1.3.11 (Fructose-Bisphosphatase)
      EC 3.1.3.2 (Phosphoric Monoester Hydrolases)
      EC 3.1.3.37 (sedoheptulose-bisphosphatase)
    • Publication Date:
      Date Created: 20080129 Date Completed: 20080623 Latest Revision: 20231213
    • Publication Date:
      20240829
    • Accession Number:
      10.1093/pcp/pcn014
    • Accession Number:
      18222961