The microtubule-based motor Kar3 and plus end-binding protein Bim1 provide structural support for the anaphase spindle.

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  • Additional Information
    • Source:
      Publisher: Rockefeller University Press Country of Publication: United States NLM ID: 0375356 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1540-8140 (Electronic) Linking ISSN: 00219525 NLM ISO Abbreviation: J Cell Biol Subsets: MEDLINE
    • Publication Information:
      Original Publication: New York : Rockefeller University Press
    • Subject Terms:
      Cell Cycle Proteins/*physiology ; Microtubule Proteins/*physiology ; Microtubule-Associated Proteins/*physiology ; Saccharomyces cerevisiae/*genetics ; Saccharomyces cerevisiae Proteins/*physiology ; Spindle Apparatus/*metabolism; Anaphase/genetics ; Anaphase/physiology ; Cell Cycle Proteins/genetics ; Chromosome Segregation ; Microtubule Proteins/genetics ; Microtubule-Associated Proteins/genetics ; Microtubules/metabolism ; Microtubules/physiology ; Models, Genetic ; Saccharomyces cerevisiae/cytology ; Saccharomyces cerevisiae/metabolism ; Saccharomyces cerevisiae Proteins/genetics ; Spindle Apparatus/genetics ; Spindle Apparatus/ultrastructure ; Tubulin/metabolism
    • Abstract:
      In budding yeast, the mitotic spindle is comprised of 32 kinetochore microtubules (kMTs) and approximately 8 interpolar MTs (ipMTs). Upon anaphase onset, kMTs shorten to the pole, whereas ipMTs increase in length. Overlapping MTs are responsible for the maintenance of spindle integrity during anaphase. To dissect the requirements for anaphase spindle stability, we introduced a conditionally functional dicentric chromosome into yeast. When centromeres from the same sister chromatid attach to opposite poles, anaphase spindle elongation is delayed and a DNA breakage-fusion-bridge cycle ensues that is dependent on DNA repair proteins. We find that cell survival after dicentric chromosome activation requires the MT-binding proteins Kar3p, Bim1p, and Ase1p. In their absence, anaphase spindles are prone to collapse and buckle in the presence of a dicentric chromosome. Our analysis reveals the importance of Bim1p in maintaining a stable ipMT overlap zone by promoting polymerization of ipMTs during anaphase, whereas Kar3p contributes to spindle stability by cross-linking spindle MTs.
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    • Grant Information:
      GM-24364 United States GM NIGMS NIH HHS; EB005568 United States EB NIBIB NIH HHS; R01 GM060678 United States GM NIGMS NIH HHS; R01 GM032238 United States GM NIGMS NIH HHS; GM-32238 United States GM NIGMS NIH HHS; R37 GM032238 United States GM NIGMS NIH HHS; GM-071522 United States GM NIGMS NIH HHS; R01 GM071522 United States GM NIGMS NIH HHS; R01 GM032238-21 United States GM NIGMS NIH HHS; F31 EB005568 United States EB NIBIB NIH HHS; R37 GM024364 United States GM NIGMS NIH HHS; R01 GM024364 United States GM NIGMS NIH HHS; P41 RR000592 United States RR NCRR NIH HHS; GM-60678 United States GM NIGMS NIH HHS; RR-00592 United States RR NCRR NIH HHS
    • Accession Number:
      0 (Ase1 protein, S cerevisiae)
      0 (BIM1 protein, S cerevisiae)
      0 (Cell Cycle Proteins)
      0 (KAR3 protein, S cerevisiae)
      0 (Microtubule Proteins)
      0 (Microtubule-Associated Proteins)
      0 (Saccharomyces cerevisiae Proteins)
      0 (Tubulin)
    • Publication Date:
      Date Created: 20080109 Date Completed: 20080208 Latest Revision: 20181113
    • Publication Date:
      20240829
    • Accession Number:
      PMC2213616
    • Accession Number:
      10.1083/jcb.200710164
    • Accession Number:
      18180364