The Possible Role of Hesperidin on Triolein Induced Lung Structural Changes in Adult Male Albino Rats (Histological and Immunohistochemical Study).

Background: Triolein used as a plasticizer, a lubricant, and present in cocoa butter. Its occupational exposure led to health hazards, especially lung injury. Hesperidin antioxidant flavonoid is present in many fruits and vegetables. The present research aimed to evaluate the possible ameliorative effect of hesperidin on triolein-induced lung structural changes in rats using histological and immunohistochemical study. Methods: Forty-five adult male albino rats were used. They were divided into Group I: (control group). Group II: rats received 0.2 ml triolein then were sacrificed after 21 days. Group III (triolein and hesperidin): rats subdivided in 2 subgroups: IIIa, IIIb, received triolein as group II and simultaneously received hesperidin 50 mg/kg/day by gavage for 21, 28 days respectively. Results: Group II showed marked affection of lung tissue. The bronchiolar epithelium showed dark-stained nuclei and vacuolated cytoplasm, desquamated epithelial cells. The alveoli collapsed with thickened interalveolar septa. The interstitial tissue showed congested blood vessels, inflammatory cellular infiltration, and extravasated blood in H&E-stained sections. Group II also showed increased deposition of collagen fibers, increased expression of alpha -Smooth muscle actin (a-SMA) in the interalveolar septa, wall of the blood vessels and bronchioles. Group IIIa, IIIb showed improvements of the previous pathological findings with more improvement in group IIIb Conclusions: hesperidin could protect the lung from the injurious effect of triolein possibly due to its anti-inflammatory, antioxidant and antifibrotic actions. [ABSTRACT FROM AUTHOR]

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