Salivary kallikrein-8 as a favorable biomarker for stress response.

Salivarni kalikrein-8 kao pogodan biomarker odgovora na stres.

Background/Aim. Kallikreins (KLKs) are a group of serine protease enzymes capable of cleaving protein peptide bonds. Besides, they are proteolytic enzymes that mediate the conversion of kininogen (alpha 2-globulin) to bradykinin or kallidin. T he aim o f t he s tudy w as t o examine whether KLK8 might serve as a novel stress biomarker. Methods. Twenty-four students (17 female and 7 male) were included in the study. The general and dental health of the students were evaluated in the appropriate anamnesis format. Unstimulated samples were collected by Sarstedt ® saliva collection tubes as recommended: 08.00-- 09.00 am, 12.00, and 2.00--3.00 pm on the exam day. KLK levels were measured by a KLK8 Human ELISA kit. Results. The salivary KLK8 levels in the morning (1.25 ± 0.26 pg/mL) were statistically significantly lower than the KLK8 levels pre-exam [at 12.00 (2.89 ± 0.85 pg/mL)] (p = 0.0006). There was also a significant difference in salivary KLK8 levels between pre- and post-exam (1.69 ± 0.39) time points (p = 0.0005). Conclusion. These results s how t hat the d ifferences i n s alivary K LK8 l evels might be related to the degree of stress, indicating that KLK8 may serve as a novel stress biomarker. [ABSTRACT FROM AUTHOR]

Uvod/Cilj. Kalikreini (KLK) su grupa serin proteaza, enzima koji su sposobni za cepanje peptidnih veza proteina. Pored toga, oni su proteolitički enzimi koji posreduju u konverziji kininogena (alfa 2-globulina) u bradikinin ili kalidin. Cilj rada bio je da se utvrdi da li bi KLK8 mogao biti novi biomarker za stres. Metode. Studijom su obuhvaćena ukupno 24 studenta (17 ženskog 7 muškog pola). Opšte i dentalno zdravlje studenata bilo je procenjivano odgovarajućom anamnezom. Nestimulisani uzorci bili su prikupljani u epruvetama za sakupljanje pljuvačke Sarstedt® na sledeći način: u 08,00--09,00, u 12,00 i u 14,00--15,00 sati na dan ispita. Nivoi KLK mereni su ELISA metodom za humani KLK8. Rezultati. Jutarnji salivarni nivoi KLK8 (1,25 ± 0,26 pg/mL) bili su statistički značajno niži od nivoa KLK8 pre ispita [u 12,00 sati (2,89 ± 0,85 pg/mL)] (p = 0,0006). Takođe postojala je značajna razlika između salivarnih nivoa KLK8 pre i posle ispita (1,69 ± 0,39) (p = 0,0005). Zaključak. Navedeni rezultati pokazuju da razlike u nivoima salivarnog KLK8 mogu biti povezane sa stepenom stresa, što ukazuje na to da KLK8 može služiti kao novi biomarker stresa. [ABSTRACT FROM AUTHOR]

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