Genome-wide epitope identification with single-amino-acid resolution via high-throughput and unbiased peptide analysis.

A preprint abstract from biorxiv.org discusses a new method called DECODE (Decoding Epitope Composition by Optimized-mRNA-display, Data analysis, and Expression sequencing) that allows for the identification of epitopes recognized by antibodies at a single amino acid resolution. This method can also predict cross-reactivity against the entire protein database. The researchers applied DECODE to serum antibodies from mice with experimental autoimmune encephalomyelitis (EAE) and successfully identified an epitope that matched the sequence of the peptide inducing the disease model. This research has the potential to improve the reproducibility of antibody-dependent experiments, diagnostics, and therapeutics, and contribute to the discovery of pathogenic epitopes from antibodies in the blood. [Extracted from the article]

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