Replication of Legionella pneumophila in floating biofilms.

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  • Author(s): Declerck P;Declerck P; Behets J; van Hoef V; Ollevier F
  • Source:
    Current microbiology [Curr Microbiol] 2007 Nov; Vol. 55 (5), pp. 435-40. Date of Electronic Publication: 2007 Aug 23.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Springer International Country of Publication: United States NLM ID: 7808448 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0343-8651 (Print) Linking ISSN: 03438651 NLM ISO Abbreviation: Curr Microbiol Subsets: MEDLINE
    • Publication Information:
      Original Publication: New York, Springer International.
    • Subject Terms:
      Biofilms*; Acanthamoeba castellanii/*microbiology ; Legionella pneumophila/*growth & development; Aeromonas hydrophila/growth & development ; Analysis of Variance ; Animals ; Escherichia coli/growth & development ; Flavobacterium/growth & development ; In Situ Hybridization, Fluorescence ; Legionella pneumophila/genetics ; Microscopy, Confocal ; Polymerase Chain Reaction/methods ; Pseudomonas aeruginosa/growth & development ; Time Factors
    • Abstract:
      Biofilms are a major source of human pathogenic Legionella pneumophila in aquatic systems. In this study, we investigated the capacity of L. pneumophila to colonize floating biofilms and the impact of Acanthamoeba castellanii on the replication of biofilm-associated Legionella. Biofilms were grown in Petri dishes and consisted of Aeromonas hydrophila, Escherichia coli, Flavobacterium breve, and Pseudomonas aeruginosa. Six hours following inoculation, Legionella were detected in floating biofilms in mean concentrations of 1.4 x 10(4) cells/cm(2 )(real-time polymerase chain reaction) and 8.3 x 10(2) CFU/cm(2 )(culture). Two-way analysis of variance tests and fluorescent in situ hybridization clearly proved that increased biofilm-associated L. pneumophila concentrations were the result of intracellular replication in A. castellanii. Forty-eight hours after the introduction of A. castellanii in the Petri dishes, 90 +/- 0.8% of the amoebae (infection rate) were completely filled with highly metabolic active L. pneumophila (mean infection intensity).
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    • Publication Date:
      Date Created: 20070824 Date Completed: 20080108 Latest Revision: 20181113
    • Publication Date:
      20221213
    • Accession Number:
      10.1007/s00284-007-9006-7
    • Accession Number:
      17713816