Metabolic flux and robustness analysis of glycerol metabolism in Klebsiella pneumoniae.

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  • Author(s): Zhang Q;Zhang Q; Teng H; Sun Y; Xiu Z; Zeng A
  • Source:
    Bioprocess and biosystems engineering [Bioprocess Biosyst Eng] 2008 Feb; Vol. 31 (2), pp. 127-35. Date of Electronic Publication: 2007 Aug 23.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101088505 Publication Model: Print-Electronic Cited Medium: Print ISSN: 1615-7591 (Print) Linking ISSN: 16157591 NLM ISO Abbreviation: Bioprocess Biosyst Eng Subsets: MEDLINE
    • Publication Information:
      Original Publication: Berlin, Germany : Springer-Verlag, 2001-
    • Subject Terms:
      Models, Biological*; Bioreactors/*microbiology ; Energy Metabolism/*physiology ; Glycerol/*metabolism ; Klebsiella pneumoniae/*metabolism ; Propylene Glycols/*metabolism ; Signal Transduction/*physiology; Cell Culture Techniques/methods ; Computer Simulation ; Metabolic Clearance Rate
    • Abstract:
      The knowledge of the mechanism of flux distribution will benefit understanding cell physiology and regulation of metabolism. In this study, the measured fluxes obtained under steady-state conditions were used to estimate intracellular fluxes and identify the robustness of branch points of the anaerobic glycerol metabolism in Klebsiella pneumoniae for the production of 1,3-propanediol by metabolic flux analysis. The biomass concentration increased as NADH(2)/NAD(+) decreased at low initial concentration and inversed at high initial glycerol concentration. The flux distribution revealed that the branch points of glycerol and dihydroxyacetonephosphate were rigid to the environmental conditions. However, the pyruvate and acetyl coenzyme A metabolisms gave cells the flexibility to regulate the energy and intermediate fluxes under various environmental conditions. Additionly, it was found that the formation rate of ethanol and the ratio of pyruvate dehydrogenase to pyruvate formate lyase appeared visible fluctuations at high glycerol uptake rate.
    • Accession Number:
      0 (Propylene Glycols)
      5965N8W85T (1,3-propanediol)
      PDC6A3C0OX (Glycerol)
    • Publication Date:
      Date Created: 20070824 Date Completed: 20080408 Latest Revision: 20131121
    • Publication Date:
      20240829
    • Accession Number:
      10.1007/s00449-007-0155-7
    • Accession Number:
      17713793