Identification and characterisation of causal pathogens of Pestalotiopsis leaf fall disease in Hevea brasiliensis using a detached leaf technique.

Pestalotiopsis leaf fall disease (PLFD) has emerged as a significant and detrimental foliar disease affecting Hevea brasiliensis in Malaysia since 2017. The disease poses a severe threat to rubber plantations, leading to economic losses and impacting the country's rubber industry. Understanding the factors contributing to lesion incidence and the potential presence of a fungal complex is crucial for effective disease management. This study aimed to address key objectives, including the identification of primary fungal pathogens responsible for lesion development and examination of the role of co-infections in lesion incidence. To achieve these objectives, five fungal isolates (Neopestalotiopsis surinamensis, Colletotrichum conoides, Lasiodiplodia theobromae, Phyllosticta fallopiae and Letendraea cordylinicola) previously identified through the Internal Transcribe Spacer (ITS) regions were subjected to in vitro artificial inoculation experiments on detached rubber leaves. Various combinations of these fungal isolates were tested to evaluate their interactions as possible causal pathogens of PLFD. Three variables were assessed, viz. type of water source (sterile distilled water and rainwater), presence of leaf wounds, and inoculation techniques (conidial suspensions and mycelial agar plugs). Lesion incidence was evaluated based on sporulating occurrences on the detached leaves, recorded seven days after inoculation. Statistical analysis, including non-parametric Pearson's chi-square tests revealed that the fungal isolates Lasiodiplodia theobromae, Colletotrichum conoides and Neopestalotiopsis surinamensis were the primary contributors to lesion incidence, accounting for 83%, 69%, and 57% of cases, respectively. The study also revealed that lesion incidence was not significantly influenced by inoculation techniques or type of water source but was greater in wounded leaves compared to unwounded leaves. Co-inoculation experiments demonstrated increased lesion incidence, highlighting the role of co-infections in disease severity. Importantly, this study elucidated a potential fungal complex associated with PLFD and provided insights into its epidemiology. The findings contribute to deeper understanding of the disease, offering valuable guidance for effective disease management strategies in H. brasiliensis cultivation in Malaysia. [ABSTRACT FROM AUTHOR]

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