High homology between variant surface glycoprotein gene expression sites of Trypanosoma brucei and Trypanosoma gambiense.

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  • Author(s): Daï Do Thi C;Daï Do Thi C; Aerts D; Steinert M; Pays E
  • Source:
    Molecular and biochemical parasitology [Mol Biochem Parasitol] 1991 Oct; Vol. 48 (2), pp. 199-210.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8006324 Publication Model: Print Cited Medium: Print ISSN: 0166-6851 (Print) Linking ISSN: 01666851 NLM ISO Abbreviation: Mol Biochem Parasitol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press.
    • Subject Terms:
      Genes* ; Sequence Homology, Nucleic Acid*; Trypanosoma brucei brucei/*genetics ; Trypanosoma brucei gambiense/*genetics ; Variant Surface Glycoproteins, Trypanosoma/*genetics; Amino Acid Sequence ; Animals ; Base Sequence ; DNA, Protozoan/chemistry ; Gene Expression ; Molecular Sequence Data ; Promoter Regions, Genetic ; Restriction Mapping ; Transcription, Genetic ; Trypanosoma brucei brucei/radiation effects ; Trypanosoma brucei gambiense/radiation effects
    • Abstract:
      The AnTat 11.17 variant surface glycoprotein (VSG) is synthesized in both metacyclic and bloodstream forms of Trypanosoma gambiense. We have characterized the AnTat 11.17 gene, and analyzed its expression site (ES) in the bloodstream form by Southern and Northern blotting with probes from the Trypanosoma brucei AnTat 1.3A VSG ES, and by run-on transcription. The AnTat 11.17 ES is located at the end of a 700-kb chromosome. It appears to contain all the genes (ESAGs, for Expression Site-Associated Genes) present in the AnTat 1.3A VSG ES, with the possible exception of ESAG 1. Limited nucleotide sequence analysis of ESAG cDNAs from the AnTat 11.17 ES shows considerable conservation with ESAGs of T. brucei. The transcription promoter of the AnTat 11.17 VSG ES, localized by virtue of the specific accumulation of promoter-proximal transcripts which occurs following UV irradiation, was found to be at the same relative position to the first ESAG (ESAG 7) as in AnTat 1.3A.
    • Molecular Sequence:
      GENBANK M61207; M61208; M62629; M62630; M62631; M62632; S74964; S74965; S74966; S78950
    • Accession Number:
      0 (DNA, Protozoan)
      0 (Variant Surface Glycoproteins, Trypanosoma)
    • Publication Date:
      Date Created: 19911001 Date Completed: 19920211 Latest Revision: 20190820
    • Publication Date:
      20231215
    • Accession Number:
      10.1016/0166-6851(91)90115-m
    • Accession Number:
      1762631