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Mosaic genome structure of echovirus type 30 that circulated in Taiwan in 2001.
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- Author(s): Chen GW;Chen GW; Huang JH; Lo YL; Tsao KC; Chang SC
- Source:
Archives of virology [Arch Virol] 2007; Vol. 152 (10), pp. 1807-17. Date of Electronic Publication: 2007 Jul 04.
- Publication Type:
Journal Article; Research Support, Non-U.S. Gov't
- Language:
English
- Additional Information
- Source:
Publisher: Springer-Verlag Country of Publication: Austria NLM ID: 7506870 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0304-8608 (Print) Linking ISSN: 03048608 NLM ISO Abbreviation: Arch Virol Subsets: MEDLINE
- Publication Information:
Original Publication: Wien, New York, Springer-Verlag.
- Subject Terms:
Disease Outbreaks* ;
Genome, Viral*;
Echovirus Infections/
*epidemiology ;
Enterovirus B, Human/
*genetics;
Amino Acid Sequence ;
Animals ;
Antibodies, Monoclonal/
immunology ;
Antibodies, Monoclonal/
metabolism ;
Base Sequence ;
Cell Line ;
Cell Line, Tumor ;
Child ;
Chlorocebus aethiops ;
Cytopathogenic Effect, Viral ;
Dogs ;
Echovirus Infections/
cerebrospinal fluid ;
Enterovirus B, Human/
classification ;
Enterovirus B, Human/
isolation & purification ;
Humans ;
Lung/
cytology ;
Lung/
virology ;
Meningitis, Aseptic/
epidemiology ;
Meningitis, Aseptic/
virology ;
Meningitis, Viral/
epidemiology ;
Molecular Sequence Data ;
Neutralization Tests ;
Phylogeny ;
RNA, Viral/
analysis ;
RNA, Viral/
isolation & purification ;
Reverse Transcriptase Polymerase Chain Reaction ;
Rhabdomyosarcoma/
pathology ;
Rhabdomyosarcoma/
virology ;
Sequence Analysis, DNA ;
Sequence Homology, Amino Acid ;
Taiwan/
epidemiology ;
Vero Cells - Abstract:
An echovirus type 30 (E30) outbreak occurred in Taiwan in 2001. In this study, one 1998 and nineteen 2001 enterovirus isolates from cerebrospinal fluid (CSF) of children with meningitis were genetically analyzed. Although negative results were obtained using the E30-specific monoclocal antibody in an immunofluorescent assay (IFA) test of all 20 isolates, molecular typing by partial VP1 sequences and subsequent neutralization test identified them as E30. Among those, seven of them were misidentified as echovirus type 4 (E4) when E4-specific monoclonal antibody was used. Complete genome sequences of one E30 isolate (TW-2513) that were IFA-positive to E4 and another (TW-3182) that was IFA-negative to both E30 and E4 were determined and analyzed. The overall percentage nucleotide identity in the structural coding region (P1) between these two isolates is 98.4, while those in the nonstructural regions P2 and P3 are only 83.2 and 84.4, respectively, indicating that the two 2001 Taiwanese E30 strains were probably recombinant. Recombination analysis of these two E30 genomes revealed that their genome structures are mosaic, which might have been formed gradually and frequently over time.
- Accession Number:
0 (Antibodies, Monoclonal)
0 (RNA, Viral)
- Publication Date:
Date Created: 20070705 Date Completed: 20071119 Latest Revision: 20191210
- Publication Date:
20231215
- Accession Number:
10.1007/s00705-007-1018-x
- Accession Number:
17610124
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