Second-Generation Bioethanol Production and Cellulases of Aspergillus niger ITV02 Using Sugarcane Bagasse as Substrate.

One of the limiting stages in the second-generation (2G) bioethanol production process is enzymatic hydrolysis due to the low availability of cellulase enzymes and their high cost. The enzymatic hydrolysis of pretreated sugarcane bagasse is optimized (Box-Behnken design) using an enzymatic extract of Aspergillus niger ITV02 cellulases produced in a bioreactor to obtain glucose. The maximum concentration of glucose (49 g/L) and conversion (70%) was reached using sugarcane bagasse with acid-alkaline pretreatment (SB-AAL) under optimal conditions of enzyme load (18 FPU/g), the concentration of substrate (84.35 g/L) and hydrolysis time (48 h). The enzymatic hydrolysate of A. niger ITV02 from SB-AAL was subsequently fermented by Saccharomyces cerevisiae ITV01 for bioethanol 2G production, reaching a yield (Yp/s) of 0.46 g/g, an efficiency of 90.77%, and 1.6 g/Lh productivity and reaching important values of parameters required for bioethanol 2G production to scale process. The present study shows that cellulases produced by A. niger ITV02 using an available and low-cost substrate such as sugarcane bagasse is efficient in the enzymatic hydrolysis of lignocellulosic residues for bioethanol 2G production. [ABSTRACT FROM AUTHOR]

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