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A role for β-1,6- and β-1,3-glucans in kinetochore function in Saccharomyces cerevisiae.
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- Author(s): Kshirsagar, Rucha; Munhoven, Arno; Nguyen, Tra My Tran; Ehrenhofer-Murray, Ann E
- Source:
Genetics. Feb2024, Vol. 226 Issue 2, p1-12. 12p. - Source:
- Additional Information
- Subject Terms:
- Abstract: Chromosome segregation is crucial for the faithful inheritance of DNA to the daughter cells after DNA replication. For this, the kinetochore, a megadalton protein complex, assembles on centromeric chromatin containing the histone H3 variant CENP-A, and provides a physical connection to the microtubules. Here, we report an unanticipated role for enzymes required for β-1,6- and β-1,3-glucan biosynthesis in regulating kinetochore function in Saccharomyces cerevisiae. These carbohydrates are the major constituents of the yeast cell wall. We found that the deletion of KRE6 , which encodes a glycosylhydrolase/ transglycosidase required for β-1,6-glucan synthesis, suppressed the centromeric defect of mutations in components of the kinetochore, foremost the NDC80 components Spc24 , Spc25 , the MIND component Nsl1 , and Okp1 , a constitutive centromere-associated network protein. Similarly, the absence of Fks1 , a β-1,3-glucan synthase, and Kre11 / Trs65 , a TRAPPII component, suppressed a mutation in SPC25. Genetic analysis indicates that the reduction of intracellular β-1,6- and β-1,3-glucans, rather than the cell wall glucan content, regulates kinetochore function. Furthermore, we found a physical interaction between Kre6 and CENP-A/ Cse4 in yeast, suggesting a potential function for Kre6 in glycosylating CENP-A/ Cse4 or another kinetochore protein. This work shows a moonlighting function for selected cell wall synthesis proteins in regulating kinetochore assembly, which may provide a mechanism to connect the nutritional status of the cell to cell-cycle progression and chromosome segregation. [ABSTRACT FROM AUTHOR]
- Abstract: Copyright of Genetics is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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