Molecular typing by pulsed-field gel electrophoresis of Flavobacterium psychrophilum isolates derived from Japanese fish.

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  • Author(s): Arai H;Arai H; Morita Y; Izumi S; Katagiri T; Kimura H
  • Source:
    Journal of fish diseases [J Fish Dis] 2007 Jun; Vol. 30 (6), pp. 345-55.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 9881188 Publication Model: Print Cited Medium: Print ISSN: 0140-7775 (Print) Linking ISSN: 01407775 NLM ISO Abbreviation: J Fish Dis Subsets: MEDLINE
    • Publication Information:
      Original Publication: Oxford, Blackwell Scientific Publications.
    • Subject Terms:
      Electrophoresis, Gel, Pulsed-Field/*veterinary ; Fish Diseases/*microbiology ; Flavobacteriaceae Infections/*veterinary ; Flavobacterium/*genetics ; Osmeriformes/*microbiology; Animals ; Cluster Analysis ; Deoxyribonucleases, Type II Site-Specific/metabolism ; Flavobacteriaceae Infections/microbiology ; Flavobacterium/classification ; Flavobacterium/isolation & purification ; Genotype ; Geography ; Japan ; Phylogeny ; Polymerase Chain Reaction/veterinary ; Polymorphism, Restriction Fragment Length
    • Abstract:
      Sixty-four isolates of Flavobacterium psychrophilum from ayu, Plecoglossus altivelis altivelis (Temminck & Schlegel), and other fish (n=16) in Japan and the type strain (NCIMB 1947(T)) were typed using pulsed-field gel electrophoresis (PFGE) with endonuclease BlnI and XhoI. These isolates were classified into 20 clusters and 42 genotypes by PFGE analysis. The most predominant cluster of isolates from ayu was cluster XII (n=20), followed by clusters XVII, XVI, XX, XI, IX, X, XIII and XV; the remaining 17 isolates from other fish were divided into clusters I, II, III, IV, V, VI, VII, VIII, XIV, XVIII and XIX. The PFGE genotype of isolates from ayu clearly differed from those of other fish. The isolates from ayu in Gunma Prefecture belonged to clusters XII, XVI, XVII and XX, and the strains of three of these clusters (XII, XVII and XX) were isolated from ayu in 15 of 19 prefectures. PFGE typing enabled more accurate classification of isolates into clusters than previously achieved by analysing the restriction fragment length polymorphism of PCR products. These results suggest that F. psychrophilum isolated from ayu and other fish are genetically different and strains with several PFGE types have spread within Japan.
    • Accession Number:
      EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific)
      EC 3.1.21.4 (GANTC-specific type II deoxyribonucleases)
      EC 3.1.21.4 (GTAC-specific type II deoxyribonucleases)
    • Publication Date:
      Date Created: 20070515 Date Completed: 20070821 Latest Revision: 20200930
    • Publication Date:
      20231215
    • Accession Number:
      10.1111/j.1365-2761.2007.00809.x
    • Accession Number:
      17498178