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Relative expression profile of the related genes with carotenoids metabolism in sweetpotato (Ipomoea batatas) based on RNA-seq data. (English)
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- Author(s): ZHAO Dong-Lan; ZHAO Ling-Xiao; LIU Yang; ZHANG An; DAI Xi-Bin; ZHOU Zhi-Lin; CAO Qing-He
- Source:
Acta Agronomica Sinica; 2023, Vol. 49 Issue 12, p3239-3249, 11p - Source:
- Additional Information
- Abstract: The objective of this study is to explore the molecular mechanism of carotenoid metabolism and color mutation of sweetpotato and to study the carotenoid metabolism related genes by RNA-seq technology. Zheshu 81 and its mutant line with color mutation in root skin (from red to yellow) and leaf vein base (from purple to green) were used as the experimental materials. Bioinformatics analysis showed that the carotenoid biosynthesis (ko00906) pathway was significantly enriched in root skin and leaf vein base. The 24 differentially expressed genes (DEGs) and 10 DEGs in the ko00906 were screened from root skin and vein base, among which four were screened in both two parts, respectively. In the upstream pathway of carotenoid anabolism, compared with Zheshu 81, the geranylgeranyl pyrophosphate synthase gene GGDPS (g29773, g38646), lycopene synthase gene PSY (g11630) and Z-carotene isomerase gene Z-ISO (g42608) were significantly up-regulated in mutant line, and GGDPS (g29773) was only expressed in mutant lines. DEGs were not screened in the upstream metabolic pathway of carotenoids in vein base. After the branching point of lycopene cyclization, two ζ-carotene hydroxylase genes CHYB (g33351, g953) were screened in root skin, one up-regulated and the other down-regulated. Two zeaxanthin epoxidase gene ZEP (g41700, g1103) were detected in root skin and vein base, respectively. And g41700 in root skin was significantly down-regulated, and g1103 in vein base was significantly up-regulated. In carotenoid catabolism, CCD8 (g21348), a carotenoid cleavage dioxygenase gene, was significantly up-regulated in root skin of mutant line. Two 9-cis epoxide carotenoid oxygenase genes NCED (g30636, g43412) were down-regulated in root skin and vein base of the mutant line. In addition, ten xanthoxin dehydrogenase ABA2 genes were detected in root skin and vein base, and nine of them were up-regulated. The relative expression levels of some genes by qRT-PCR were highly consistent with those of RNA-seq data. This study provides an important reference for the molecular mechanism of carotenoid metabolism and the analysis of different color mutations in root skin and vein base of sweetpotato. [ABSTRACT FROM AUTHOR]
- Abstract: Copyright of Acta Agronomica Sinica is the property of Crop Science Society of China and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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