MT-بررسي اثر تمرين هوازی و مکملياری با روغن تخم کدو و عصاره نخود سفید بر بیان ژن 1 در بافت کبد موشهای صحرايي نر cGPDH و MT-2 (Persian).

Investigating the Effect of Aerobic Exercise and Supplementation with Pumpkin Seed Oil and White Pea Extract on the Expression of MT-1, MT-2, and cGPDH Genes in the Liver Tissue of Male Rats. (English)

ZINC metabolism; IRON metabolism; REVERSE transcriptase polymerase chain reaction; AEROBIC exercises; ESSENTIAL oils; PUMPKINS; LIVER; ANIMAL experimentation; DIETARY supplements; GENE expression; RATS; SEEDS; TISSUES; DESCRIPTIVE statistics; PLANT extracts; DATA analysis software

Background and Aim: The lack of micronutrients, including iron and zinc, are one of the major concerns of today that can negatively affect sports performance. Therefore, the purpose of this research is to investigate the effect of aerobic exercise and supplementation with pumpkin seed oil and white pea extract on the gene expression levels of MT-1, MT-2, and cGPDH in the liver tissue of male rats. Methods: In this experimental research, 36 male Wistar rats with a weight range of 130 to 150 g were randomly divided into six groups: saline control, saline training, pumpkin seed oil control, training + pumpkin seed oil, Control pea, and training + pea. The training groups participated in the aerobic training with an intensity of 25 m/min, 5 days a week, for total time of 6 weeks. Twenty-four hours after the last training session, the liver tissue of rats was extracted and kept at -80 °C for the subsequent measurement changes of MT-1, MT-2, and cGPDH genes gene expression levels through real-time PCR method as well as zinc and Iron levels through ICPEOS technique in the liver tissue. Statistical analysis of data was done with SPSS software and one-way analysis of variance and Tukey's post hoc test. Results: Six weeks of training + pumpkin seed oil supplementation significantly increased gene expression of MT-1, MT-2, as well as zinc and iron levels in liver tissue in comparison to the control group (P≤0.05). Also, the amount of liver zinc and iron in the training group + pumpkin oil was significantly higher compared to the training group + pea group (P≤0.05). On the other hand, there was no difference in the expression level of the GPDH gene in liver tissue among the groups (P≤0.05). Conclusion: The results of the present study showed that the use of supplements containing zinc increases the amount of zinc and subsequently increases zinc transporters. However, pumpkin supplement is more effective in MT1, and MT2 gene expression compared to chickpea supplement. [ABSTRACT FROM AUTHOR]

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