Synergistic protection by Araloside A with L-ascorbic acid on oxidative stress via Nrf2/CAT activation in HEK293 cells.

[Display omitted] • Araloside A and L-ascorbic acid had a synergistic antioxidant effect. • Araloside A and L-ascorbic acid synergistically increased Nrf2 nuclear translocation. • The combination of Araloside A and L-ascorbic acid elevated the expression of CAT. • Nrf2/CAT pathway was synergistically activated by Araloside A and L-ascorbic acid. The synergistic effect of different antioxidants has potential application prospect in alleviating oxidative injury. Araloside A is the most abundant active ingredient of Aralia elata (Miq.) Seem, which exhibits multiple biological activities, including antioxidant, et al. This study investigated the interaction between Araloside A and several natural antioxidants, and explored the mechanism of the combination with synergistic antioxidant effect. The results showed that only the combination of Araloside A and L-ascorbic acid (A + Vc) exhibited synergistic effects in alleviating oxidative damage. A + Vc enhanced the expression levels of NF-E2-related factor 2 (Nrf2) and Catalase (CAT) in a time-dependent and dose-dependent manner. However, the Nrf2 inhibitor-ML385 and CAT inhibitor-Pyocyanin impaired the protective effect of A + Vc. To conclude, A + Vc could ameliorate H 2 O 2 -induced oxidative injury via synergistic antioxidative effects originated from Nrf2/CAT pathway activation. Hence, dietary intake of A + Vc may be a practical, safe approach to prevent diseases associated with oxidative damage. [ABSTRACT FROM AUTHOR]

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