鼠尾草酸调节CXCR7/CXCL12轴对胃癌AGS细胞增殖、迁移和侵袭 能力的影响. (Chinese)

Carnosic acid affects the proliferation, migration, and invasion of gastric cancer AGS cells by regulating CXCR7/CXCL12 axis. (English)

Objective: To investigate the effects of carnosic acid (CA) on the proliferation, migration, and invasion of gastric cancer cells by regulating CXC chemokine receptor 7 (CXCR7)/CXC chemokine ligand 12 (CXCL12) axis. Methods: CCK-8 method was used to select appropriate concentrations of CA. Gastric cancer AGS cells were treated with CA at different concentrations (0, 5, 10, 20, 40, 80 µg/mL). AGS cells were divided into the control group (untreated AGS cells), CA group (20 µg/mL CA treatment), CA+ siCXCR7 group (siCXCR7 transfection+20 µg/mL CA treatment), CA+siNC group (siNC transfection+20 µg/mL CA treatment), CA+ vectorNC group (vectorNC transfection+20 µg/mL CA treatment), and CA+vectorCXCR7 group (vectorCXCR7 transfection+20 µg/ mL CA treatment). The proliferation of AGS cells was detected by CCK-8 method; the expression levels of CXCR7 and CXCL12 mRNA in cells were detected by qPCR; and the invasion of cells was detected by Transwell assay; the change of cell migration ability was detected by scratch assay, and the expressions of cyclin D1, Bcl-2, CXCR7, CXCL12 and MMP-2 were detected by WB assay. Results: Different concentrations of CA were all able to inhibit the survival rate of AGS cells. When the concentration was 20 µg/mL, the survival rate of AGS cells was closed to 50%. 20 µg/mL CA was therefore chosen for subsequent research. Compared with the control group, the proliferation rate, invasion number, migration rate, cyclin D1, MMP-2, Bcl-2, CXCR7, CXCL12 mRNA and protein expression of the CA group decreased significantly (all P<0.05). Compared with the CA+siNC group, the proliferation rate, invasion number, migration rate, cyclin D1, MMP-2, Bcl-2, CXCR7, CXCL12 mRNA and protein expression in the CA+siCXCR7 group decreased significantly (all P<0.05). Compared with the CA+vectorNC group, the proliferation rate, invasion number, migration rate, cyclin D1, MMP-2, Bcl-2, CXCR7, CXCL12 mRNA and protein expression in the CA+vectorCXCR7 group increased significantly (all P<0.05). Conclusion: CA can inhibit the proliferation, migration, and invasion of AGS cells, which may be related to the inhibition of CXCR7/CXCL12 axis. [ABSTRACT FROM AUTHOR]

目的: 探讨鼠尾草酸 (CA) 通过调节CXC基序趋化因子受体7 (CXCR7) /CXC基序趋化因子配体 (CXCL12) 轴对胃癌 AGS细胞增殖, 迁移和侵袭的影响. 方法: 用不同浓度 (0, 5, 10, 20, 40, 80 µg/mL) ) 的CA处理胃癌AGS细胞, 采用CCK-8法筛 选合适的 CA 浓度; 将 AGS 细胞分为对照组 (未经处理的 AGS 细胞), CA 组 (20 µg/mL CA 处理), CA+siCXCR7 组 (转染 siCXCR7+20 µg/mL CA 处理), CA+siNC 组 (转染 siNC+20 µg/mL CA 处理), CA+vectorNC 组 (转染 vectorNC+20 µg/mL CA 处 理), CA+vectorCXCR7组 (转染vectorCXCR7+20 µg/mL CA处理), 采用CCK-8法检测AGS细胞增殖的变化, qPCR法检测细胞 中CXCR7, CXCL12 mRNA表达水平的变化, Transwell实验检测细胞侵袭能力的变化, 划痕实验检测细胞迁移能力的变化, WB 法检测周期蛋白D1, Bcl-2, CXCR7, CXCL12, MMP-2蛋白表达的变化. 结果: 不同浓度CA均可抑制AGS细胞存活率, 且浓度 为20 µg/mL时, 细胞存活率接近50%, 故选择20 µg/mL CA用于后续研究. 与对照组相比, CA组增殖率, 侵袭数, 迁移率, 周期蛋 白D1, MMP-2, Bcl-2, CXCR7, CXCL12 mRNA及蛋白表达显著降低 (均P<0.05) ; 与CA+siNC组相比, CA+siCXCR7组增殖率, 侵 袭数, 迁移率, 周期蛋白D1, MMP-2, Bcl-2, CXCR7, CXCL12 mRNA及蛋白表达显著降低 (均P<0.05) ; 与CA+vectorNC组相比, CA+vectorCXCR7组增殖率, 侵袭数, 迁移率, 周期蛋白 D1, MMP-2, Bcl-2, CXCR7, CXCL12 mRNA 及蛋白表达显著增加 (均 P<0.05) . 结论: CA可抑制AGS细胞增殖, 迁移和侵袭, 其机制可能与抑制CXCR7/CXCL12轴有关. [ABSTRACT FROM AUTHOR]

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