Involvement of NMDA receptor mechanisms in the modulation of serotonin release in the lateral parabrachial nucleus in the rat.

Publisher: Elsevier Science Country of Publication: United States NLM ID: 7605818 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0361-9230 (Print) Linking ISSN: 03619230 NLM ISO Abbreviation: Brain Res Bull Subsets: MEDLINE

Publication: New York Ny : Elsevier Science
Original Publication: Phoenix, N. Y. ANKHO International Inc.

Pons/*metabolism ; Receptors, N-Methyl-D-Aspartate/*metabolism ; Serotonin/*metabolism ; Synaptic Transmission/*physiology ; Visceral Afferents/*metabolism ; Water-Electrolyte Balance/*physiology; Angiotensin-Converting Enzyme Inhibitors/pharmacology ; Animals ; Diuretics/pharmacology ; Drinking/drug effects ; Drinking/physiology ; Excitatory Amino Acid Agonists/pharmacology ; Excitatory Amino Acid Antagonists/pharmacology ; Extracellular Fluid/drug effects ; Extracellular Fluid/metabolism ; Glutamic Acid/metabolism ; Hydroxyindoleacetic Acid/metabolism ; Male ; Microdialysis ; Pons/drug effects ; Presynaptic Terminals/drug effects ; Presynaptic Terminals/metabolism ; Rats ; Rats, Wistar ; Receptors, N-Methyl-D-Aspartate/drug effects ; Sodium, Dietary/metabolism ; Synaptic Transmission/drug effects ; Thirst/drug effects ; Thirst/physiology ; Visceral Afferents/drug effects ; Water-Electrolyte Balance/drug effects

Microdialysis was employed to investigate whether N-methyl-d-asparatate (NMDA) glutamate receptor mechanisms are involved in the modulation of serotonin (5-hydoxytryptamine, 5-HT) release in the region of the lateral parabrachial nucleus (LPBN) in freely moving rats. Perfusion of NMDA (10 and 50 microM) through the microdialysis probe significantly enhanced extracellular concentrations of 5-HT and its metabolite 5-hydroxyindoleacetic acid (5-HIAA) in the LPBN area. Local perfusion of the NMDA antagonist dizocilpine (MK801, 10 and 50 microM) did not change the basal 5-HT and 5-HIAA levels in the LPBN area. MK801 (10 microM) administered together with NMDA antagonized the stimulant effect of NMDA (10 microM). The intake of 0.3M NaCl and water induced by subcutaneous injections of the diuretic furosemide (FURO, 10 mg/kg) and the angiotensin converting enzyme inhibitor captopril (CAP, 5 mg/kg) produced significant increases in the 5-HT and 5-HIAA concentrations in the LPBN area. The increased levels of 5-HT and 5-HIAA caused by the combined treatment with FURO and CAP were attenuated by perfusion of MK801 (10 microM). These results indicate the participation of NMDA receptors in the control of 5-HT release in the LPBN area.

0 (Angiotensin-Converting Enzyme Inhibitors)
0 (Diuretics)
0 (Excitatory Amino Acid Agonists)
0 (Excitatory Amino Acid Antagonists)
0 (Receptors, N-Methyl-D-Aspartate)
0 (Sodium, Dietary)
333DO1RDJY (Serotonin)
3KX376GY7L (Glutamic Acid)
54-16-0 (Hydroxyindoleacetic Acid)

Date Created: 20061123 Date Completed: 20070201 Latest Revision: 20181201

20221213

10.1016/j.brainresbull.2006.09.017

17113961