Relationships among the biosyntheses of ubiquinone, carotene, sterols, and triacylglycerols in Zygomycetes.

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  • Author(s): Kuzina V;Kuzina V; Domenech C; Cerdá-Olmedo E
  • Source:
    Archives of microbiology [Arch Microbiol] 2006 Dec; Vol. 186 (6), pp. 485-93. Date of Electronic Publication: 2006 Sep 29.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 0410427 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0302-8933 (Print) Linking ISSN: 03028933 NLM ISO Abbreviation: Arch Microbiol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Berlin, New York, Springer-Verlag.
    • Subject Terms:
      Ergosterol/*biosynthesis ; Mucorales/*metabolism ; Phycomyces/*metabolism ; Triglycerides/*biosynthesis ; Ubiquinone/*biosynthesis ; beta Carotene/*biosynthesis; Acetyl Coenzyme A/metabolism ; Carbon Radioisotopes ; Leucine/metabolism ; Mevalonic Acid/metabolism ; Multivariate Analysis
    • Abstract:
      The Zygomycetes Phycomyces blakesleeanus and Blakeslea trispora are actual or potential sources of beta-carotene, ergosterol, ubiquinone, edible oil, and other compounds. By feeding [14C]acetyl-CoA, L-[14C]leucine, or R-[14C]mevalonate in the presence of excess unlabeled glucose, we found that ubiquinone (the terpenoid moiety), beta-carotene, and triacylglycerols were made from separate pools of all their common intermediates; the pools for ubiquinone and ergosterol were indistinguishable. Fatty acids were not labeled from mevalonate, showing the absence in these fungi of a shunt pathway that would recycle carbon from mevalonate and its products back to central metabolism. The overproduction of carotene in a Phycomyces mutant and in sexually mated cultures of Blakeslea modified the relative use of labeled and unlabeled carbon sources in the production of carotene, but not of the other compounds. We concluded that carotene, ubiquinone, and triacylglycerols are synthesized in separate subcellular compartments, while sterols and ubiquinone are synthesized in the same compartments or in compartments that exchange precursors. Carotene biosynthesis was regulated specifically and not by flow diversion in a branched pathway.
    • Accession Number:
      0 (Carbon Radioisotopes)
      0 (Triglycerides)
      01YAE03M7J (beta Carotene)
      1339-63-5 (Ubiquinone)
      72-89-9 (Acetyl Coenzyme A)
      GMW67QNF9C (Leucine)
      S5UOB36OCZ (Mevalonic Acid)
      Z30RAY509F (Ergosterol)
    • Publication Date:
      Date Created: 20060930 Date Completed: 20070220 Latest Revision: 20131121
    • Publication Date:
      20231215
    • Accession Number:
      10.1007/s00203-006-0166-9
    • Accession Number:
      17009023