[Synthesis of new gene-loaded microbubbles serve as gene delivery vehicle applied in reporter gene transfer into cardiac myocytes].

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  • Author(s): Wang G;Wang G; Hu S; Zheng Z; Sun J; Zheng X; Zhu Z; Li J; Yao Y
  • Source:
    Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi [Sheng Wu Yi Xue Gong Cheng Xue Za Zhi] 2006 Aug; Vol. 23 (4), pp. 856-61.
  • Publication Type:
    English Abstract; Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    Chinese
  • Additional Information
    • Source:
      Publisher: Sichuan Sheng sheng wu yi xue gong cheng xue hui Country of Publication: China NLM ID: 9426398 Publication Model: Print Cited Medium: Print ISSN: 1001-5515 (Print) Linking ISSN: 10015515 NLM ISO Abbreviation: Sheng Wu Yi Xue Gong Cheng Xue Za Zhi Subsets: MEDLINE
    • Publication Information:
      Publication: Chengdu : Sichuan Sheng sheng wu yi xue gong cheng xue hui
      Original Publication: Chengdu : Sichuan Sheng sheng wu yi xue gong cheng xue hui : Hua xi yi ke da xue : Chengdu ke ji da xue,
    • Subject Terms:
      Genetic Vectors* ; Microbubbles* ; Sonication*; Genes, Reporter/*genetics ; Transfection/*methods ; beta-Galactosidase/*genetics; Animals ; Cells, Cultured ; Imines ; Myocytes, Cardiac/metabolism ; Plasmids/genetics ; Polyethylenes ; Rats ; Rats, Wistar ; beta-Galactosidase/biosynthesis
    • Abstract:
      To improve the stability and gene-carried capability of gene-attached microbubbles, the method for manufacture of albumin microbubbles was modified and new gene-loaded microbubbles were synthesized by incorporated gene-PEI complex into the shell of microbubbles. Agarose gel electrophoresis and bacteria transformation showed that PEI had the ability to provide the protection of plasmid DNA from ultrasonic degradation. The new gene-loaded microbubbles exhibited excellent acoustical and hemorheological properties. Moreover, they could carry more plasmid DNA than gene-attached microbubbles. beta-galactosidase plasmid transfection into cardiac myocytes was performed by using ultrasound targeted destruction of new gene-loaded microbubbles or gene-attached microbubbles. Gene expression in cardiac myocytes was detected by beta-galactosidase in situ staining and quantitive assay. It was shown that beta-galactosidase activity in cardiac myocytes was enhanced 107-fold by ultrasonic destruction of gene-loaded microbubbles compared with naked plasmid transfection and new gene-loaded microbubbles resulted in 6.85-fold increase in beta-galactosidase activity compared with optimal transfection mediated by gene-attached microbubbles. These results suggested that ultrasonic destruction of the gene-loaded microbubbles can enhance the cardiac myocytes exogenous gene transfer efficiency significantly and new gene-loaded microbubbles is an efficient and safe gene delivery vehicle.
    • Accession Number:
      0 (Imines)
      0 (Polyethylenes)
      0 (poly(ethylene imine))
      EC 3.2.1.23 (beta-Galactosidase)
    • Publication Date:
      Date Created: 20060928 Date Completed: 20070531 Latest Revision: 20060927
    • Publication Date:
      20221213
    • Accession Number:
      17002125