A novel sex-specific DNA marker in Columbidae birds.

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  • Author(s): Wu CP;Wu CP; Horng YM; Wang RT; Yang KT; Huang MC
  • Source:
    Theriogenology [Theriogenology] 2007 Jan 15; Vol. 67 (2), pp. 328-33. Date of Electronic Publication: 2006 Sep 11.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: United States NLM ID: 0421510 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0093-691X (Print) Linking ISSN: 0093691X NLM ISO Abbreviation: Theriogenology Subsets: MEDLINE
    • Publication Information:
      Publication: [New York, N.Y.?] : Elsevier
      Original Publication: Los Altos, Calif., Geron-X.
    • Subject Terms:
      Genetic Markers*; Columbidae/*genetics ; Random Amplified Polymorphic DNA Technique/*veterinary ; Sex Determination Analysis/*veterinary; Animals ; Base Sequence ; DNA/chemistry ; DNA Fingerprinting/veterinary ; Female ; Gene Amplification ; Male ; Molecular Sequence Data ; Random Amplified Polymorphic DNA Technique/methods ; Sequence Alignment/veterinary ; Sex Characteristics ; Species Specificity
    • Abstract:
      That most Columbidae birds have no conspicuous sexual dimorphism often makes it difficult to identify their sex on the basis of external morphology. In the present study, we report a novel sex-specific DNA marker in Columbidae birds. DNA was extracted from one member of this bird group, Streptopelia orientalis (S. orientalis, oriental turtle dove), and used to identify a female-specific DNA marker using a random amplified polymorphic DNA (RAPD) fingerprinting. One hundred and sixty random primers were used for the RAPD-PCR reactions. When using the OPAV17 primer, a novel 902 bp sex-specific PCR product was amplified from known female birds. This fragment of DNA was cloned and sequenced. Two primers, TurSexOPAV17-F and TurSexOPAV17-R, were designed from the cloned sex-specific sequence, and were successfully used to amplify a 777 bp female-specific fragment using PCR from S. orientalis DNA. This sex-specific marker was also amplified from genomic DNA samples of two other female Columbidae, S. chinensis and Columba livia. Sequence analysis showed that this novel sex-specific marker was highly conserved amongst these three bird species. In contrast, the PCR product was not amplified from male DNA of these species, nor from either sex of the S. chinensis formosa birds. Therefore, we concluded that our novel marker can be used to rapidly and accurately identify the sex of birds from three species of Columbidae.
    • Molecular Sequence:
      GENBANK DQ219457
    • Accession Number:
      0 (Genetic Markers)
      9007-49-2 (DNA)
    • Publication Date:
      Date Created: 20060913 Date Completed: 20071031 Latest Revision: 20101118
    • Publication Date:
      20240829
    • Accession Number:
      10.1016/j.theriogenology.2006.08.001
    • Accession Number:
      16965809