Transforming growth factor beta 1 modulates extracellular matrix organization and cell-cell junctional complex formation during in vitro angiogenesis.

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  • Additional Information
    • Source:
      Publisher: Wiley-Liss Country of Publication: United States NLM ID: 0050222 Publication Model: Print Cited Medium: Print ISSN: 0021-9541 (Print) Linking ISSN: 00219541 NLM ISO Abbreviation: J Cell Physiol Subsets: MEDLINE
    • Publication Information:
      Publication: New York, NY : Wiley-Liss
      Original Publication: Philadelphia, Wistar Institute of Anatomy and Biology.
    • Subject Terms:
      Endothelium, Vascular/*cytology ; Extracellular Matrix/*drug effects ; Intercellular Junctions/*drug effects ; Neovascularization, Pathologic/*pathology ; Transforming Growth Factors/*physiology; Animals ; Cell Communication/drug effects ; Cell Division/drug effects ; Cells, Cultured ; Endothelium, Vascular/drug effects ; Endothelium, Vascular/ultrastructure ; Extracellular Matrix/ultrastructure ; Intercellular Junctions/ultrastructure ; Microscopy, Electron ; Organelles/ultrastructure ; Rats ; Rats, Inbred Strains
    • Abstract:
      Transforming growth factor-beta 1 (TGF-beta 1) is angiogenic in vivo. In two-dimensional (2-D) culture systems microvascular endothelial cell proliferation is inhibited up to 80% by TGF-beta 1; however, in three-dimensional (3-D) collagen gels TGF-beta 1 is found to have no effect on proliferation while eliciting the formation of calcium and magnesium dependent tube-like structures mimicking angiogenesis. DNA analyses performed on 3-D cell cultures reveal no significant difference in the amount of DNA or cell number in control versus TGF-beta 1 treated cultures. In 2-D cultures TGF-beta 1 is known to increase cellular fibronectin accumulation; however, in 3-D cultures no difference is seen between control and TGF-beta 1 treated cells as established by ELISA testing for type IV collagen, fibronectin, and laminin. In 3-D cultures there is increased synthesis and secretion of type V collagen in both control and TGF-beta 1 treated cultures over 2-D cultures. Even though an equal amount of type V collagen is seen in both 3-D conditions, there is a reorganization of the protein with concentration along an organizing basal lamina in TGF-beta 1 treated cultures. EM morphological analyses on 3-D cultures illustrate quiescent, control cells lacking cell contacts. In contrast, TGF-beta 1 treated cells show increased pseudopod formation, cell-cell contact, and organized basal lamina-like material closely apposed to the "abluminal" plasma membranes. TGF-beta 1 treated cells also appear to form junctional complexes between adjoining cells. Immunofluorescence using specific antibodies to the tight junction protein ZO-1 results in staining at apparent cell-cell junctions in the 3-D cultures. Northern blots of freshly isolated microvascular endothelium, 2-D and 3-D cultures, using cDNA and cRNA probes specific for the ZO-1 tight junction protein, reveal the presence of the 7.8 kb mRNA. Western blots of rat epididymal fat pad endothelial cells (RFC) monolayer lysates probed with anti-ZO-1 label a 220 kd band which co-migrates with the bonafide ZO-1 protein. These data confirm and support the hypothesis that TGF-beta 1 is angiogenic in vitro, eliciting microvascular endothelial cells to form tube-like structures with apparent tight junctions and abluminal basal lamina deposition in three-dimensional cultures.
    • Grant Information:
      DK 34989 United States DK NIDDK NIH HHS; P01 DK 38979 United States DK NIDDK NIH HHS; R01-HL-28373 United States HL NHLBI NIH HHS
    • Accession Number:
      76057-06-2 (Transforming Growth Factors)
    • Publication Date:
      Date Created: 19900101 Date Completed: 19900228 Latest Revision: 20071114
    • Publication Date:
      20231215
    • Accession Number:
      10.1002/jcp.1041420115
    • Accession Number:
      1688859