Enhancement of xylitol productivity and yield using a xylitol dehydrogenase gene-disrupted mutant of Candida tropicalis under fully aerobic conditions.

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  • Author(s): Ko BS;Ko BS; Rhee CH; Kim JH
  • Source:
    Biotechnology letters [Biotechnol Lett] 2006 Aug; Vol. 28 (15), pp. 1159-62. Date of Electronic Publication: 2006 Jun 30.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Kluwer Academic Publishers Country of Publication: Netherlands NLM ID: 8008051 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0141-5492 (Print) Linking ISSN: 01415492 NLM ISO Abbreviation: Biotechnol Lett Subsets: MEDLINE
    • Publication Information:
      Publication: 1999- : Dordrecht : Kluwer Academic Publishers
      Original Publication: [Kew, Eng., Science and Technology Letters]
    • Subject Terms:
      Candida tropicalis/*enzymology ; Candida tropicalis/*genetics ; D-Xylulose Reductase/*metabolism ; Glycerol/*metabolism ; Xylitol/*biosynthesis; Aerobiosis/physiology ; Candida tropicalis/metabolism ; Industrial Microbiology/methods ; Kinetics ; Oxygen/metabolism
    • Abstract:
      The effects of glycerol and the oxygen transfer rate on the xylitol production rate by a xylitol dehydrogenase gene (XYL2)-disrupted mutant of Candida tropicalis were investigated. The mutant produced xylitol near the almost yield of 100% from D: -xylose using glycerol as a co-substrate for cell growth and NADPH regeneration: 50 g D: -xylose l(-1) was completely converted into xylitol when at least 20 g glycerol l(-1) was used as a co-substrate. The xylitol production rate increased with the O(2) transfer rate until saturation and it was not necessary to control the dissolved O(2) tension precisely. Under the optimum conditions, the volumetric productivity and xylitol yield were 3.2 g l(-1) h(-1) and 97% (w/w), respectively.
    • Accession Number:
      EC 1.1.1.9 (D-Xylulose Reductase)
      PDC6A3C0OX (Glycerol)
      S88TT14065 (Oxygen)
      VCQ006KQ1E (Xylitol)
    • Publication Date:
      Date Created: 20060701 Date Completed: 20061207 Latest Revision: 20131121
    • Publication Date:
      20221213
    • Accession Number:
      10.1007/s10529-006-9068-9
    • Accession Number:
      16810450