The isolation of nuclei and basic nucleoproteins from the cellular slime mold Dictyostelium discoideum.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read Online Read More Add to Saved list
  • Author(s): Charlesworth MC; Parish RW
  • Source:
    European journal of biochemistry [Eur J Biochem] 1975 May; Vol. 54 (1), pp. 307-16.
  • Publication Type:
    Comparative Study; Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies Country of Publication: England NLM ID: 0107600 Publication Model: Print Cited Medium: Print ISSN: 0014-2956 (Print) Linking ISSN: 00142956 NLM ISO Abbreviation: Eur J Biochem Subsets: MEDLINE
    • Publication Information:
      Publication: -2004: Oxford, UK : Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
      Original Publication: Berlin, New York, Springer.
    • Subject Terms:
      Dictyostelium/*ultrastructure ; Myxomycetes/*ultrastructure ; Nucleoproteins/*isolation & purification; Animals ; Cattle ; Cell Fractionation ; Cell Nucleus/analysis ; Densitometry ; Dictyostelium/analysis ; Electrophoresis, Polyacrylamide Gel ; Ficoll ; Histones ; Perchlorates ; Polyethylene Glycols ; Solubility ; Sorbitol ; Thymus Gland
    • Abstract:
      A method is described for the isolation of nuclei from an axenic strain of Dictyostelium discoideum using a sorbitol/Ficoll solution and low concentration of Triton X-100. Basic proteins have been extracted from the nuclei and on polyacrylamide gel electrophoresis yield a consistent pattern in which five major groups or bands predominate. Four of these five fractions comigrate with calf thymus histones and one fraction seems to be unique to D. discoideum. The slowest moving of the five fractions is soluble in 0.5 M perchloric acid and comigrates with calf thymus histone F1. After recovery from the perchloric acid solution by precipitation with acetone this fraction yielded one major band on electrophoresis.
    • Accession Number:
      0 (Histones)
      0 (Nucleoproteins)
      0 (Perchlorates)
      25702-74-3 (Ficoll)
      3WJQ0SDW1A (Polyethylene Glycols)
      506T60A25R (Sorbitol)
    • Publication Date:
      Date Created: 19750501 Date Completed: 19751108 Latest Revision: 20190620
    • Publication Date:
      20240829
    • Accession Number:
      10.1111/j.1432-1033.1975.tb04141.x
    • Accession Number:
      168080