The effects of estradiol on estrogen receptor and glutamate transporter expression in organotypic hippocampal cultures exposed to oxygen--glucose deprivation.

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  • Additional Information
    • Source:
      Publisher: Kluwer Academic/Plenum Publishers Country of Publication: United States NLM ID: 7613461 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0364-3190 (Print) Linking ISSN: 03643190 NLM ISO Abbreviation: Neurochem Res Subsets: MEDLINE
    • Publication Information:
      Publication: 1999- : New York, NY : Kluwer Academic/Plenum Publishers
      Original Publication: New York, Plenum Press
    • Subject Terms:
      Hypoxia*; Estradiol/*metabolism ; Estrogen Receptor alpha/*metabolism ; Estrogen Receptor beta/*metabolism ; Excitatory Amino Acid Transporter 1/*metabolism ; Excitatory Amino Acid Transporter 2/*metabolism ; Glucose/*metabolism ; Hippocampus/*cytology; Animals ; Cells, Cultured ; Hippocampus/metabolism ; Male ; Rats ; Rats, Wistar
    • Abstract:
      The molecular basis of estrogen-mediated neuroprotection against brain ischemia remains unclear. In the present study, we investigated changes in expression of estrogen receptors (ERs) alpha and beta and excitatory amino acid transporters (EAAT) 1 and 2 in rat organotypic hippocampal slice cultures treated with estradiol and subsequently exposed to oxygen--glucose deprivation (OGD). Pretreatment with 17beta-estradiol (10 nM) for 7 days protected the CA1 area of hippocampus against OGD (60 min), reducing cellular injury by 46% compared to the vehicle control group. Levels of ERalpha protein were significantly reduced by 20% after OGD in both vehicle- and estradiol-treated cultures, whereas ERbeta was significantly up-regulated by 25% in the estradiol-treated cultures. In contrast, EAAT1 and EAAT2 levels were unchanged in response to estradiol treatment in this model of OGD. These findings suggest that estrogen-induced neuroprotection against ischemia might involve regulation of ERbeta and, consequently, of the genes influenced by this receptor.
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    • Accession Number:
      0 (Estrogen Receptor alpha)
      0 (Estrogen Receptor beta)
      0 (Excitatory Amino Acid Transporter 1)
      0 (Excitatory Amino Acid Transporter 2)
      0 (Slc1a2 protein, rat)
      0 (Slc1a3 protein, rat)
      4TI98Z838E (Estradiol)
      IY9XDZ35W2 (Glucose)
    • Publication Date:
      Date Created: 20060608 Date Completed: 20070322 Latest Revision: 20181113
    • Publication Date:
      20240829
    • Accession Number:
      10.1007/s11064-006-9043-9
    • Accession Number:
      16758356