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Inhibition of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) endocytosis by ouabain in human endothelial cells.
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- Author(s): Trevisi L;Trevisi L; Pighin I; Bazzan S; Luciani S
- Source:
FEBS letters [FEBS Lett] 2006 May 15; Vol. 580 (11), pp. 2769-73. Date of Electronic Publication: 2006 Apr 24.
- Publication Type:
Journal Article; Research Support, Non-U.S. Gov't
- Language:
English
- Additional Information
- Source:
Publisher: John Wiley & Sons Ltd Country of Publication: England NLM ID: 0155157 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0014-5793 (Print) Linking ISSN: 00145793 NLM ISO Abbreviation: FEBS Lett Subsets: MEDLINE
- Publication Information:
Publication: Jan. 2016- : West Sussex : John Wiley & Sons Ltd.
Original Publication: Amsterdam, North-Holland on behalf of the Federation of European Biochemical Societies.
- Subject Terms:
- Abstract:
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) uptake and reduction is widely used to evaluate cell proliferation and viability. MTT is taken up by the cells through endocytosis. We find that ouabain (1-200 nM) inhibits MTT reduction in human umbilical vein endothelial cells (HUVEC) without affecting cell viability. Ouabain does not inhibit MTT reduction when cell lysates substituted for the intact cells. Disruption of caveolae by cholesterol depletion, completely prevents the effect of ouabain. Treatment of HUVEC with Src inhibitor 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine partially abrogates the inhibitory effect of ouabain. The data suggest that ouabain interaction with caveolar Na/K-ATPase inhibits MTT endocytosis through the activation of signaling proteins such as Src kinase.
- Accession Number:
0 (Phosphoinositide-3 Kinase Inhibitors)
0 (Protein Kinase Inhibitors)
0 (Tetrazolium Salts)
0 (Thiazoles)
5ACL011P69 (Ouabain)
97C5T2UQ7J (Cholesterol)
EC 2.7.10.2 (src-Family Kinases)
EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
EUY85H477I (thiazolyl blue)
- Publication Date:
Date Created: 20060502 Date Completed: 20060705 Latest Revision: 20191210
- Publication Date:
20231215
- Accession Number:
10.1016/j.febslet.2006.04.040
- Accession Number:
16647703
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