Effect of sympathectomy and demedullation on increased myenteric and dorsal vagal complex Fos-like immunoreactivity by cholecystokinin-8.

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  • Additional Information
    • Source:
      Publisher: Elsevier/North Holland Country of Publication: Netherlands NLM ID: 8100479 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0167-0115 (Print) Linking ISSN: 01670115 NLM ISO Abbreviation: Regul Pept Subsets: MEDLINE
    • Publication Information:
      Original Publication: [Amsterdam] : Elsevier/North Holland, 1980-
    • Subject Terms:
      Sympathectomy, Chemical*; Adrenal Medulla/*physiology ; Myenteric Plexus/*physiology ; Proto-Oncogene Proteins c-fos/*physiology ; Sincalide/*pharmacology ; Vagus Nerve/*physiology; Adrenal Medulla/surgery ; Animals ; Guanethidine ; Male ; Myenteric Plexus/drug effects ; Proto-Oncogene Proteins c-fos/immunology ; Rats ; Rats, Sprague-Dawley ; Vagus Nerve/drug effects
    • Abstract:
      Chemical sympathectomy with daily, intraperitoneal (IP) injections of guanethidine sulfate to adult rats, attenuated myenteric, but not dorsal vagal complex (DVC) Fos-like immunoreactivity (Fos-LI) by cholecystokinin-8 (CCK). This technique destroys only 60-70% of the sympathetic neurons, and spares the hormonal source of catecholamines, the adrenal medulla. The goal of the current study is to evaluate the effect of complete sympathectomy or destroying 100% of the sympathetic neurons by injecting guanethidine to 1-day-old pups (40 mg/kg daily for 5 weeks), and surgically removing the adrenal medulla. In the DVC, demedullation and sympathectomy-demedullation increased Fos-LI by CCK in the area postrema and nucleus of the solitary tract, but sympathectomy-demedullation increased it only in the area postrema. In the myenteric plexus, sympathectomy increased this response in the duodenum, and demedullation increased it in the duodenum and jejunum. On the other hand, sympathectomy-demedullation attenuated myenteric Fos-LI in the jejunum. These results indicate that catecholamines may play an inhibitory role on the activation of the DVC neurons by CCK. In the myenteric neurons, however, catecholamines may have both inhibitory and excitatory roles depending on the level of the intestine e.g., duodenum vs. jejunum. This may also indicate that CCK activates the enteric neurons by different mechanisms or through different pathways.
    • Grant Information:
      S06/GM08091-31 United States GM NIGMS NIH HHS
    • Accession Number:
      0 (Proto-Oncogene Proteins c-fos)
      M03GIQ7Z6P (Sincalide)
      ZTI6C33Q2Q (Guanethidine)
    • Publication Date:
      Date Created: 20060408 Date Completed: 20060804 Latest Revision: 20131121
    • Publication Date:
      20221213
    • Accession Number:
      10.1016/j.regpep.2006.02.006
    • Accession Number:
      16600401