Identification of a High-Molecular-Weight Glycoconjugate from a Uruguayan Plant that Binds to the Tumor-Associated Tn Antigen.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read Online Read More Add to Saved list
  • Additional Information
    • Subject Terms:
      GLYCOCONJUGATES; CARBOHYDRATES; TUMOR antigens; MEDICINAL plants; MOLECULAR weights
    • Subject Terms:
      URUGUAY
    • Abstract:
      The Tn determinant (GalNAc-O-Ser=Thr), one of the most specific human carcinoma-associated structures, can be identified using both monoclonal antibodies and lec-tins. In this work we have explored, for the first time, the presence of Tn-binding proteins in aqueous extracts of plants collected in Uruguay. A total of 21 extracts from plants belonging to 12 Phanerogam families were screened by an ELISA assay (competitive inhibition of anti-Tn antibody binding) and by hemagglutination (HAG) activity. Five of the extracts displayed binding to Tn residues, although only those extracts prepared from fruit and leaf from Myrsine coriacea (Sw.) R. Br. ex Roem & Schult caused total inhibition of the binding activity of anti-Tn MAb 83D4. Consi-dering that Myrsine coriacea extracts did not display HAG activity against several types of normal red blood cells, we characterized the material with Tn-binding activity from these extracts. We purified this material from Myrsine coriacea leaf extracts using perc-hloric acid treatment followed by affinity chromatography and reverse-phase high performance liquid chromatography (HPLC), conserving its Tnbinding activity. We found a high-molecular-weight glycoconjugate that has an apparent molecular mass of approximately 670 kDa, as judged by SDS-PAGE. [ABSTRACT FROM AUTHOR]
    • Abstract:
      Copyright of Pharmaceutical Biology is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)