Advanced techniques in placental biology -- workshop report.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 8006349 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0143-4004 (Print) Linking ISSN: 01434004 NLM ISO Abbreviation: Placenta Subsets: MEDLINE
    • Publication Information:
      Publication: <2010- > : Amsterdam : Elsevier
      Original Publication: London W.B. Saunders.
    • Subject Terms:
      MicroRNAs/*physiology ; Microdissection/*methods ; Micromanipulation/*methods ; Microscopy, Immunoelectron/*methods ; Placenta/*physiology ; Protein Array Analysis/*methods; Animals ; Cryoultramicrotomy/methods ; Female ; Fluorescent Antibody Technique ; Gene Expression Regulation ; Humans ; Lymphocytes/metabolism ; Micromanipulation/instrumentation ; Uterus/metabolism
    • Abstract:
      Major advances in placental biology have been realized as new technologies have been developed and existing methods have been refined in many areas of biological research. Classical anatomy and whole-organ physiology tools once used to analyze placental structure and function have been supplanted by more sophisticated techniques adapted from molecular biology, proteomics, and computational biology and bioinformatics. In addition, significant refinements in morphological study of the placenta and its constituent cell types have improved our ability to assess form and function in highly integrated manner. To offer an overview of modern technologies used by investigators to study the placenta, this workshop: Advanced techniques in placental biology, assembled experts who discussed fundamental principles and real time examples of four separate methodologies. Y. Sadovsky presented the principles of microRNA function as an endogenous mechanism of gene regulation. J. Robinson demonstrated the utility of correlative microscopy in which light-level and transmission electron microscopy are combined to provide cellular and subcellular views of placental cells. A. Croy provided a lecture on the use of microdissection techniques which are invaluable for isolating very small subsets of cell types for molecular analysis. Finally, G. Rice presented an overview methods on profiling of complex protein mixtures within tissue and/or fluid samples that, when refined, will offer databases that will underpin a systems approach to modern trophoblast biology.
    • Accession Number:
      0 (MicroRNAs)
    • Publication Date:
      Date Created: 20060321 Date Completed: 20060926 Latest Revision: 20181201
    • Publication Date:
      20240829
    • Accession Number:
      10.1016/j.placenta.2006.01.012
    • Accession Number:
      16546252