The cotranscribed Salmonella enterica sv. Typhi tsx and impX genes encode opposing nucleoside-specific import and export proteins.

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  • Author(s): Bucarey SA;Bucarey SA; Villagra NA; Fuentes JA; Mora GC
  • Source:
    Genetics [Genetics] 2006 May; Vol. 173 (1), pp. 25-34. Date of Electronic Publication: 2006 Feb 19.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Oxford University Press Country of Publication: United States NLM ID: 0374636 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0016-6731 (Print) Linking ISSN: 00166731 NLM ISO Abbreviation: Genetics Subsets: MEDLINE
    • Publication Information:
      Publication: 2021- : [Oxford] : Oxford University Press
      Original Publication: Austin, Tex. [etc.]
    • Subject Terms:
      Adenosine/*metabolism ; Bacterial Outer Membrane Proteins/*genetics ; Genes, Bacterial/*genetics ; Salmonella enterica/*genetics ; Salmonella enterica/*metabolism ; Salmonella typhi/*genetics ; Salmonella typhi/*metabolism ; Transcription, Genetic/*drug effects; DNA, Bacterial/biosynthesis ; Epistasis, Genetic ; Mutation/genetics ; Protein Transport/genetics ; Salmonella enterica/classification ; Salmonella typhi/classification ; Suppression, Genetic
    • Abstract:
      The Salmonella enterica tsx gene encodes a nucleoside-specific outer membrane channel. The Tsx porin is essential for the prototrophic growth of S. enterica sv. Typhi in the absence of nucleosides. RT-PCR analysis shows that the tsx gene is cotranscribed with an open reading frame unique to S. enterica, impX (STY0450), which encodes an inner membrane protein 108 amino acids in length, which is predicted to have only two transmembrane alpha-helices. Fusions of the lacZ gene to both tsx and impX reveal that the transcription of both genes is induced in the presence of adenosine. A null mutation in the S. Typhi impX gene suppresses the induced auxotrophy for adenosine or thymidine resulting from a tsx mutation and confers sensitivity to high concentrations of adenosine or thymidine. The ImpX protein, when tagged with a 3xFLAG epitope, is functional and associates with the inner membrane; impX mutants are defective in the export of 3H-radiolabeled thymidine. Taken together, these and other results suggest that the S. Typhi Tsx porin and ImpX inner membrane protein facilitate competing mechanisms of thymidine influx and efflux, respectively, to maintain the steady-state levels of internal nucleoside pools.
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    • Accession Number:
      0 (Bacterial Outer Membrane Proteins)
      0 (DNA, Bacterial)
      K72T3FS567 (Adenosine)
    • Publication Date:
      Date Created: 20060221 Date Completed: 20060731 Latest Revision: 20181113
    • Publication Date:
      20240829
    • Accession Number:
      PMC1461456
    • Accession Number:
      10.1534/genetics.105.054700
    • Accession Number:
      16489221