Prediction value of radiosensitivity of hepatocarcinoma cells for apoptosis and micronucleus assay.

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  • Additional Information
    • Source:
      Publisher: Baishideng Publishing Group Country of Publication: United States NLM ID: 100883448 Publication Model: Print Cited Medium: Print ISSN: 1007-9327 (Print) Linking ISSN: 10079327 NLM ISO Abbreviation: World J Gastroenterol Subsets: MEDLINE
    • Publication Information:
      Publication: 2014- : Pleasanton, CA : Baishideng Publishing Group
      Original Publication: Beijing : WJG Press, c1998-
    • Subject Terms:
      Apoptosis* ; Carcinoma, Hepatocellular* ; Liver Neoplasms* ; Micronucleus Tests* ; Radiation Tolerance*; Cell Line, Tumor/*physiology ; Cell Line, Tumor/*radiation effects; Cell Line, Tumor/cytology ; Cell Survival ; Dose-Response Relationship, Radiation ; Humans ; Micronuclei, Chromosome-Defective ; Predictive Value of Tests ; X-Rays
    • Abstract:
      Aim: To investigate the prediction value of radiosensitivity of hepatocarcinoma cells for apoptosis and micronucleus assay.
      Methods: Clonogenic assay, flow cytometry, and CB micronuclei assay were used to survey the cell survival rate, radiation-induced apoptosis and micronucleus frequency of hepatocarcinoma cell lines SMMC-7721, HL-7702, and HepG2 after being irradiated by X-ray at the dosage ranging 0-8 Gy.
      Results: After irradiation, there was a dose-effect relationship between micronucleus frequency and radiation dosage among the three cell lines (P<0.05). A positive relationship was observed between apoptosis and radiation dosage among the three cell lines. The HepG2 cells had a significant correlation (P<0.05) but apoptosis incidence had a negative relationship with micronucleus frequency. There was a positive relationship between apoptosis and radiation dosage and the correlation between SMMC-7721 and HL-7702 cell lines had a significant difference (P<0.01). After irradiation, a negative relationship between cell survival rate and radiation dosages was found among the three cell lines (P<0.01). There was a positive relationship between cell survival rate and micronucleus frequency (P<0.01). No correlation was observed between apoptosis and cell survival rate.
      Conclusion: The radiosensitivity of hepatocarcinoma cells can be reflected by apoptosis and micronuclei. Detection of apoptosis and micronuclei could enhance the accuracy for predicting radiosensitivity.
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    • Publication Date:
      Date Created: 20060127 Date Completed: 20060509 Latest Revision: 20190430
    • Publication Date:
      20221213
    • Accession Number:
      PMC4717051
    • Accession Number:
      10.3748/wjg.v11.i44.7036
    • Accession Number:
      16437613