Effects of Resveratrol on Pulmonary Fibrosis via TGF-β/Smad/ERK Signaling Pathway.

INFLAMMATION prevention; DRUG efficacy; IN vitro studies; COLLAGEN; GLUTATHIONE; CYTOKINES; REVERSE transcriptase polymerase chain reaction; STATISTICS; FIBROBLASTS; ANIMAL experimentation; LUNGS; WESTERN immunoblotting; SUPEROXIDE dismutase; ANTIOXIDANTS; MICRORNA; RESVERATROL; CELLULAR signal transduction; RATS; PEROXIDASE; GENE expression; T-test (Statistics); PROLINE; MESSENGER RNA; ENZYME-linked immunosorbent assay; DESCRIPTIVE statistics; RESEARCH funding; PULMONARY fibrosis; MOLECULAR structure; DATA analysis software; DATA analysis

Pulmonary fibrosis (PF) is a progressive pulmonary disease with no effective treatment and high mortality. Resveratrol has shown promising benefits in the treatment of PF. However, the probable efficacy and underlying mechanism of resveratrol in PF treatment remain unclear. This study investigates the intervention effects and potential mechanisms underpinning the treatment of PF with resveratrol. The histopathological analysis of lung tissues in PF rats showed that resveratrol improved collagen deposition and reduced inflammation. Resveratrol decreased the levels of collagen, glutathione, superoxide dismutase, myeloperoxidase, and hydroxyproline, lowered total anti-oxidant capacity, and suppressed the migration of TGF- β 1 and LPS-induced 3T6 fibroblasts. With resveratrol intervention, the protein and RNA expressions of TGF- β 1, a-SMA, Smad3/4, p-Smad3/4, CTGF, and p-ERK1/2 were markedly downregulated. Similarly, the protein and RNA expression levels of Col-1 and Col-3 were significantly downregulated. However, Smad7 and ERK1/2 were evidently upregulated. The protein and mRNA expression levels of TGF- β , Smad, and p-ERK correlated positively with the lung index, while the protein and mRNA expression levels of ERK correlated negatively with the lung index. These results reveal that resveratrol may have therapeutic effects on PF by reducing collagen deposition, oxidation, and inflammation. The mechanism is associated with the regulation of the TGF- β /Smad/ERK signaling pathway. [ABSTRACT FROM AUTHOR]

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