Restraint of IFN-γ expression through a distal silencer CNS–28 for tissue homeostasis.

Interferon-γ (IFN-γ) is a key cytokine in response to viral or intracellular bacterial infection in mammals. While a number of enhancers are described to promote IFN-γ responses, to the best of our knowledge, no silencers for the Ifng gene have been identified. By examining H3K4me1 histone modification in naive CD4+ T cells within Ifng locus, we identified a silencer (CNS–28) that restrains Ifng expression. Mechanistically, CNS–28 maintains Ifng silence by diminishing enhancer-promoter interactions within Ifng locus in a GATA3-dependent but T-bet-independent manner. Functionally, CNS–28 restrains Ifng transcription in NK cells, CD4+ cells, and CD8+ T cells during both innate and adaptive immune responses. Moreover, CNS–28 deficiency resulted in repressed type 2 responses due to elevated IFN-γ expression, shifting Th1 and Th2 paradigm. Thus, CNS–28 activity ensures immune cell quiescence by cooperating with other regulatory cis elements within the Ifng gene locus to minimize autoimmunity. [Display omitted] • Identification of the Ifng silencer CNS–28 • CNS–28 represses interaction between CNS–22 and Ifng promoter • CNS–28 maintains tissue quiescence by restraining IFN-γ production • CNS–28 deletion represses type 2 responses via enhanced IFN-γ expression There are multiple enhancer regions for the Ifng locus, but Ifng restraint is unknown. Cui et al. identify a silencer CNS–28 that diminishes enhancer-promoter interactions within Ifng locus in a GATA3-dependent but T-bet-independent manner. Together with other regulatory elements, CNS–28 activity ensures immune cell quiescence and minimizes autoimmunity. [ABSTRACT FROM AUTHOR]

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