Invasive phenotype and apoptosis induction of Plesiomonas shigelloides P-1 strain to Caco-2 cells.

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  • Additional Information
    • Source:
      Publisher: Oxford University Press Country of Publication: England NLM ID: 9706280 Publication Model: Print Cited Medium: Print ISSN: 1364-5072 (Print) Linking ISSN: 13645072 NLM ISO Abbreviation: J Appl Microbiol Subsets: MEDLINE
    • Publication Information:
      Publication: 2022- : Oxford : Oxford University Press
      Original Publication: Oxford : Published for the Society for Applied Bacteriology by Blackwell Science, c1997-
    • Subject Terms:
      Caco-2 Cells/*microbiology ; Gram-Negative Bacterial Infections/*transmission ; Plesiomonas/*pathogenicity; Apoptosis ; Bacterial Adhesion ; Bacteriological Techniques ; Caco-2 Cells/pathology ; Flow Cytometry ; Gram-Negative Bacterial Infections/microbiology ; Humans ; In Situ Nick-End Labeling ; Time Factors ; Virulence
    • Abstract:
      Aims: The mechanism of the host cell invasion of Plesiomonas shigelloides and its capability to induce apoptosis were investigated.
      Methods and Results: We performed a time course experiment on the bacterial adherence and invasion of the P. shigelloides P-1 strain into Caco-2 cells using an invasion assay and flow cytometry. The adherence of P. shigelloides to the Caco-2 cells was almost completed within 10 min after the infection. Thereafter, P. shigelloides starts internalization within the Caco-2 cells, which was completed within 60 min after the infection. Based on the invasion assay using nocodazole, cytochalasin D, and genistein, it became clear that the mechanism of the internalization depended on the signal transduction followed by the rearrangement of the cytoskeletal protein. Based on the DNA laddering and TUNEL methods, the cytotoxicity of the Caco-2 cells by the invasion of P. shigelloides occurred through the induction of apoptosis.
      Conclusions: This work demonstrated that the mechanism of invasion of P. shigelloides into Caco-2 cells and the invasion of P. shigelloides induces apoptotic cell death.
      Significance and Impact of the Study: This work revealed the virulence factor, which may be important for understanding of the pathogenesis of P. shigelloides.
    • Publication Date:
      Date Created: 20051130 Date Completed: 20060306 Latest Revision: 20051129
    • Publication Date:
      20231215
    • Accession Number:
      10.1111/j.1365-2672.2005.02721.x
    • Accession Number:
      16313416