高浓度CO2肋、迫对软枣称猴桃贮藏期间细胞壁代谢的影响 . (Chinese)

Effects of High CO2 Stress on Cell Wall Metabolism of Actinidia arguta during Storage. (English)

Abstract To investigate the effect of high CO2 stress on cell wall metabolism of A ctinidia arguta. Based on the tongcheng NO. 2' as the experimental material, the air—regulated element A (CK2) and air—regulated element B (TR) are respectively pasted, and the air—regulated element CK1 is not pasted, research during the high concentration of CO2 stress on frozen fruit peel strength, cell wall polysaccharides, cell wall degradation enzymes and reactive oxygen species (ROS), the microstructure of fruit peel in different treatment groups was analyzed on day 60. The results showed that the suitable storage environment (02 content 17.00%-20.92%, CO2 content 1.63%-2.78%) was formed in CK2 group during cold storage. TR group formed a high concentration of CO2 stress storage environment (02 content is 11.22%— 15.55%, CO2 content is 9.00%-11.93%). Compared with CK1 and CK2, TR group could inhibit the decrease of peel strength, affect the normal decrease of propectin, cellulose and hemicellulose contents, inhibit the increase of water—soluble pectin, and decrease the activities of polygalacturonase (PG), cellulase (Cs), β —galactosidase (β—Gal) and amylase. The activity of superoxide anion (O2), content of hydrogen peroxide (H202), malondialdehyde (MDA) and relative electrical conductivity were increased. At the same time, the structure of pericarp cells was seriously damaged, the epi-dermis was rough and disorderly, the cell gap was enlarged, and the tissue contour was blurred and disordered. Further analysis showed that the CO2 content in the TR group exceeded the tolerance threshold during storage, which resulted in the slow decline of fruit rigidity and hardness, affected the process of post—ripening, inhibited the metabolic transformation of cell wall polysaccharide, the accumulation of free radicals in the tissue membrane lipid peroxidation, damaged cell structure, and lost commercial property. Keywords Act india arguta; CO2 damage; cell wall; ultrastructure [ABSTRACT FROM AUTHOR]

为研究贮藏期间高浓度CO2 胁迫时软冬称猴桃细胞壁代谢的影响，以“龙成二号”为试验材料，以微环境气调箱为载 体，分别粘贴气调元件A(CK2)、气调元件B (TR)，以不粘贴气调元件为CKI，研究高浓度CO2 胁迫对冷藏期间果实果皮强 度、细胞壁多糖、细胞壁降解酶及活性氧(ROS)的影响。在60 d时分析不同处理组果实果皮微观结构的差异。结果表明：冷 藏过程中，CK2组中形成适于软冬称猴桃贮藏的环境（0：含量1 7.(X)%-20.92%,CO2含量1 .63%-2.78%)，而TR组形成高 浓度CO2胁迫的贮藏环境（O2含量1 1.22%-15.55%,CO2含量9.00%-I 1.93%)。与CK1和CK2相比，TR组可阻碍果皮强度 的降低，影响原果胶、纤维素、半纤维素含量的正常下降，抑制水溶性果胶的升高，降低多聚半乳糖醛酸酶(PG)、纤维素酶 (C、）、月一半乳糖等酶（刀-Gal）及淀粉酶活性，加快超氧阴离子（O2）活性、过氧化氢(H刃2)、丙二醛(MDA）含量及相对电导率 的上升；同时果皮细胞结构破坏严重，表皮粗糙凌乱，细胞间隙增大，组织轮廓模糊紊乱。分析表明，Til组软冬称猴桃贮藏 期间环境CO2含量超过耐受闪值，引起果实僵直，硬度下降缓慢，影响后熟进程，细胞壁多糖代谢转化受到抑制，组织内自 由基大量积累，膜脂过氧化，细胞结构被破坏，丧失商品性。 [ABSTRACT FROM AUTHOR]

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