A newly identified 50 kDa protein, which is associated with phosphodiesterase 3B, is phosphorylated by insulin in rat adipocytes.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Author(s): Onuma H;Onuma H; Osawa H; Ogura T; Tanabe F; Nishida W; Makino H
  • Source:
    Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2005 Nov 25; Vol. 337 (3), pp. 976-82. Date of Electronic Publication: 2005 Oct 03.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0006-291X (Print) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE
    • Publication Information:
      Publication: <2002- >: San Diego, CA : Elsevier
      Original Publication: New York, Academic Press.
    • Subject Terms:
      3',5'-Cyclic-AMP Phosphodiesterases/*chemistry ; 3',5'-Cyclic-AMP Phosphodiesterases/*metabolism ; Adipocytes/*metabolism ; Insulin/*administration & dosage; Adipocytes/drug effects ; Animals ; Cells, Cultured ; Cyclic Nucleotide Phosphodiesterases, Type 3 ; Dose-Response Relationship, Drug ; Molecular Weight ; Phosphorylation/drug effects ; Protein Binding ; Rats ; Rats, Sprague-Dawley ; Signal Transduction/physiology
    • Abstract:
      Phosphodiesterase 3B (PDE3B), a major PDE isoform in adipocytes, plays a pivotal role in the anti-lipolytic action of insulin. Insulin phosphorylates and activates PDE3B in a phosphatidylinositol 3-kinase-dependent manner. We identified a new 50 kDa protein that is phosphorylated by insulin and is co-immunoprecipitated with PDE3B by anti-PDE3B antibodies in rat adipocytes. The insulin-induced phosphorylation of the 50 kDa protein was also detected in a cell free system against the N-terminal and the catalytic regions, which are more than 700 amino acids apart recognize the 50 kDa protein, suggesting that it is not a proteolytic product, but an associated protein with PDE3B. Phosphoamino acid analysis indicated that both serine and threonine residues in the 50 kDa protein were phosphorylated, but only serine residues in PDE3B were phosphorylated. Therefore, it appears likely that this is a new protein which is associated with PDE3B.
    • Accession Number:
      0 (Insulin)
      EC 3.1.4.17 (3',5'-Cyclic-AMP Phosphodiesterases)
      EC 3.1.4.17 (Cyclic Nucleotide Phosphodiesterases, Type 3)
      EC 3.1.4.17 (Pde3b protein, rat)
    • Publication Date:
      Date Created: 20051018 Date Completed: 20051215 Latest Revision: 20111117
    • Publication Date:
      20240829
    • Accession Number:
      10.1016/j.bbrc.2005.09.144
    • Accession Number:
      16225849