[In vitro study on cranial neural crest differentiating into ectomesenchymal cell of the first branchial arch by FGF-8].

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Author(s): Jiang HB;Jiang HB; Tian WD; Tang W; Liu L; Li XD
  • Source:
    Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology [Zhonghua Kou Qiang Yi Xue Za Zhi] 2005 Jul; Vol. 40 (4), pp. 319-22.
  • Publication Type:
    English Abstract; Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    Chinese
  • Additional Information
    • Source:
      Publisher: Chinese Medical Association Country of Publication: China NLM ID: 8711066 Publication Model: Print Cited Medium: Print ISSN: 1002-0098 (Print) Linking ISSN: 10020098 NLM ISO Abbreviation: Zhonghua Kou Qiang Yi Xue Za Zhi Subsets: MEDLINE
    • Publication Information:
      Publication: Beijing : Chinese Medical Association
      Original Publication: Beijing : Zhonghua yi xue hui, 1987-
    • Subject Terms:
      Cell Differentiation/*drug effects ; Cranial Nerves/*cytology ; Fibroblast Growth Factor 8/*pharmacology ; Mesoderm/*cytology ; Neural Crest/*cytology; Animals ; Branchial Region/cytology ; Cells, Cultured ; Female ; Male ; Mice ; Mice, Inbred Strains
    • Abstract:
      Objective: To investigate the effects of FGF-8 on cranial neural crest cell (CNCC) differentiating into ectomesenchymal cell of the first branchial arch, and determine the appropriate dose and stage of CNCC exposure to FGF-8.
      Methods: Cranial neural crest explants were cultured in free-serum medium containing modified DMEM/F12 and different doses of FGF-8. The differentiation type of CNCC were determined by in situ hybridization for Hoxa2 and immunocytochemistry for vimentin.
      Results: Pre-emigrating CNCC demonstrated the negative Hoxa2 stain and positive vimentin stain after treated by 100 ug/FGF-8. Both post-emigrating CNCC group and control group were positive for Hoxa2 and vimentin stain.
      Conclusions: On the early stage of CNCC emigration, the first branchial arch phenotype of CNCC could be induced by FGF-8. This experiment could provide in vitro model for study on the mechanism of tooth-jaw regeneration.
    • Accession Number:
      148997-75-5 (Fibroblast Growth Factor 8)
    • Publication Date:
      Date Created: 20050930 Date Completed: 20091029 Latest Revision: 20050929
    • Publication Date:
      20231215
    • Accession Number:
      16191378