[Purification of lipase from latex of Euphorbia characias by an extraction method with apolar solvent].

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  • Author(s): Moulin A;Moulin A; Giordani R; Teissère M; Piéroni G
  • Source:
    Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie [C R Acad Sci III] 1992; Vol. 314 (8), pp. 337-42.
  • Publication Type:
    English Abstract; Journal Article
  • Language:
    French
  • Additional Information
    • Transliterated Title:
      Purification d'une lipase dans le latex d'Euphorbia characias par une méthode d'extraction en solvant apolaire.
    • Source:
      Publisher: Éditions Scientifiques et Médicales Elsevier Country of Publication: France NLM ID: 8503078 Publication Model: Print Cited Medium: Print ISSN: 0764-4469 (Print) Linking ISSN: 07644469 NLM ISO Abbreviation: C R Acad Sci III Subsets: MEDLINE
    • Publication Information:
      Publication: 1997- : Paris : Éditions Scientifiques et Médicales Elsevier
      Original Publication: Paris : Gauthier-Villars, c1984-c2001.
    • Subject Terms:
      Latex/*chemistry ; Lipase/*isolation & purification; Chromatography, Thin Layer/methods ; Electrophoresis, Polyacrylamide Gel ; Plant Extracts/analysis ; Solvents
    • Abstract:
      A lipase from the latex of Euphorbia characias was purified using a new method involving extraction by apolar solvent and adsorption chromatography on silica. The lipase (specific activity 1,500 IU/mg of protein) was eluted from silica complexed with a lipid. The main proteic fraction, showing a molecular weight of 38,000, was inactive when dissociated from the lipid fraction. It was necessary to reassociate the lipid and proteic fractions for 72% of the lipolytic activity to be recovered.
    • Accession Number:
      0 (Latex)
      0 (Plant Extracts)
      0 (Solvents)
      EC 3.1.1.3 (Lipase)
    • Publication Date:
      Date Created: 19920101 Date Completed: 19920805 Latest Revision: 20061115
    • Publication Date:
      20240829
    • Accession Number:
      1617480