Asymmetric photocross-linking of singly phosphorylated smooth muscle heavy meromyosin.

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  • Additional Information
    • Source:
      Publisher: Oxford University Press Country of Publication: England NLM ID: 0376600 Publication Model: Print Cited Medium: Print ISSN: 0021-924X (Print) Linking ISSN: 0021924X NLM ISO Abbreviation: J Biochem Subsets: MEDLINE
    • Publication Information:
      Publication: : Abingdon, UK : Oxford University Press
      Original Publication: Tokyo : Japanese Biochemical Society
    • Subject Terms:
      Cross-Linking Reagents/*metabolism ; Muscle, Smooth/*metabolism ; Myosin Subfragments/*metabolism ; Protein Isoforms/*metabolism; Amino Acid Sequence ; Animals ; Benzophenones/chemistry ; Benzophenones/metabolism ; Chickens ; Light ; Models, Molecular ; Molecular Sequence Data ; Myosin Subfragments/chemistry ; Phosphorylation ; Protein Isoforms/chemistry
    • Abstract:
      Although activities of smooth muscle myosin are regulated by phosphorylation, the molecular mechanisms of regulation have not been fully established. Phosphorylation of both heads of myosin is known to activate ATPase and motor activities, but the effects of phosphorylation of only one of the heads have not been established. Such information on singly phosphorylated myosin can serve to elucidate the molecular mechanism of the phosphorylation-dependent regulation. To understand the structural properties of the singly phosphorylated state, we prepared singly phosphorylated heavy meromyosin (HMM) containing a photoreactive benzophenone-labeled RLC and examined its photocross-linking reactivity. The two heads in the singly phosphorylated HMM showed different reactivities. The dephosphorylated RLC in the singly phosphorylated HMM was cross-linked to a heavy chain, like that in the dephosphorylated HMM, whereas the phosphorylated RLC did not react, like that in the fully phosphorylated HMM. These results indicate that the two heads of the singly phosphorylated HMM have an asymmetric structure, suggesting that phosphorylation of one head can to some extent activate smooth muscle HMM.
    • Accession Number:
      0 (Benzophenones)
      0 (Cross-Linking Reagents)
      0 (Myosin Subfragments)
      0 (Protein Isoforms)
      701M4TTV9O (benzophenone)
    • Publication Date:
      Date Created: 20050920 Date Completed: 20051202 Latest Revision: 20121115
    • Publication Date:
      20221213
    • Accession Number:
      10.1093/jb/mvi122
    • Accession Number:
      16169875