高效液相色谱法测定追风活血膏中8种成分. (Chinese)

Determination of 8 components in Zhuifeng Huoxue Ointment by high performance liquid chromatography. (English)

A high performance liquid chromatographic （HPLC） method was developed for determination of the eight components in Zhuifeng Huoxue Ointment, namely, brucine, aconitine, strychnine, angelica, frankincense, myrrh, ecdysterone and sinomenine. The chromatographic column was an Agilent ZORBAXSB-C18 column （4.6 mm×250 mm, 5 μm）; the mobile phase was methanol （phase A） and 0.1% aqueous solution of H3PO4 （phase B）; the flow rate was 1.0 mL/min; the column temperature was room temperature; the detection wavelength was 256 nm. The average recoveries ranged from 98.74%-99.67%, and the relative standard deviations （RSDs） were in the range of 0.85%-1.76%. The content of strychnine, aconitine, brucine, angelica, frankincense, myrrh, ecdysterone and sinomenine in the five batches of samples were 0.031-0.349, 0.021-0.166, 0.024-0.298, 0.021-0.219, 0.017-0.186, 0.015-0.158, 0.015-0.149, 0.016-0.179 mg/g, respectively. The HPLC method developed is simple, stable and controllable, and can be used for the quality control of Zhuifeng Huoxue Ointment. [ABSTRACT FROM AUTHOR]

建立了高效液相色谱（HPLC）测定追风活血膏中士的宁、乌头碱、马钱子碱、当归、乳香、没药、蜕皮甾酮和 青藤碱8种成分含量的方法。色谱柱为Agilent ZORBAXSB-C18柱（4.6 mm×250 mm, 5 μm）;流动相：甲醇（A相）, 0.1% H3PO4水溶液（B相）;流速1.0 mL/min;柱温室温;检测波长256 nm。结果显示, 8种成分能够达到良好分离, 在各自 线性范围内线性关系良好, 相关系数r均大于0.999;平均加样回收率在98.74%~99.67%之间, 相对标准偏差（RSD）在 0.85%~1.76%范围内。5批次样品中士的宁、乌头碱、马钱子碱、当归、乳香、没药、蜕皮甾酮和青藤碱的含量分别为 0.031~0.349, 0.021~0.166, 0.024~0.298, 0.021~0.219, 0.017~0.186, 0.015~0.158, 0.015~0.149, 0.016~0.179 mg/g。本 文所建立的HPLC法操作简单、稳定可控, 可用于追风活血膏的质量控制. [ABSTRACT FROM AUTHOR]

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