Resveratrol Supplementation Attenuates Excessive Inflammation and Helps Restore Impaired Restitution in an Intestinal Epithelial Cell Culture Model.

Aim: Sustained release of inflammatory mediators, excessive inflammatory response and impaired intestinal epithelial restitution are wellknown mechanisms in the pathogenesis of necrotizing enterocolitis. This study focused on the effect of resveratrol on these pathways. Materials and Methods: In this study, the rat crypt intestinal cell line (IEC-6) culture, an application of lipopolysaccharide or a cytokine mixture and a scratch migration assay model were used. Nitric oxide synthase-2 (iNOS) and cyclooxygenase-2 (COX-2), focal adhesion kinase (FAK) and its phosphorylated form (pFAK) levels were assessed. Results: IEC-6 cells covered 88% of the denuded area in the control, 54% in LPS, and 35% in cytomix groups at the 24th hour. The treatment with resveratrol at doses of 0.5, 1 and 5 μM/L before LPS resulted in the repair of 84%, 87% and 76% of the denuded areas, respectively. Likewise, with cytomix, it was 86%, 82%, and 78%. Resveratrol at a dose of 5 μM/L prevented an increase in iNOS levels. All three doses of resveratrol were effective in preventing increases in COX levels. FAK or pFAK expressions remained unchanged in all groups. Conclusion: Resveratrol, being known for its antioxidant features, suppresses excessive inflammatory response and helps preservation of mucosal integrity by conservation of epithelial restitution. [ABSTRACT FROM AUTHOR]

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