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Advanced hybridoma technology for selective production of high-affinity monoclonal antibodies through B-cell receptors.
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- Author(s): Sakashita, Kento1,2 (AUTHOR) ; Tsumoto, Kanta1 (AUTHOR); Tomita, Masahiro1 (AUTHOR)
- Source:
Journal of Immunological Methods. Dec2022, Vol. 511, pN.PAG-N.PAG. 1p.- Subject Terms:
- Source:
- Additional Information
- Abstract: In general, it is difficult to raise novel monoclonal antibodies against relatively low-molecular weight antigen, and particularly those with high homology for the mouse protein. The optimized B-cell targeting (BCT) technique can overcome this limitation. The point of this advanced technology is the selection of sensitized B lymphocytes by the antigen through B-cell receptors (BCRs). This strict selection by specific and strong interaction between antigen and antibody enables the efficient production of monoclonal antibodies with high specificity and affinity. It also offers the condensation of sensitized target B lymphocytes to selectively generate hybridoma cells secreting desired monoclonal antibodies. In this study, several kinds of biotinylated human myoglobin (hMyo) were prepared to select sensitized B lymphocytes via BCRs. Biotinylated hMyo prepared by a 3.75- and 7.5-fold molar excess of N -hydroxysuccinimide (NHS)-biotin provided high antigenicity of 68–88%. B lymphocytes selected by these biotinylated antigens had an ELISA-positive rate >17 times higher than that with usual biotinylated antigen. Monoclonal antibodies generated by the optimized BCT technology by preselecting sensitized B lymphocytes with the target antigen were identified to specifically recognize lower antigenic epitopes in hMyo with high affinity, while this would be impossible by the polyethylene glycol (PEG) method. Furthermore, combination of these high-affinity monoclonal antibodies gave the best binding rate in an epitope binning assay. These outcomes could be attributed to the unique characteristic that BCRs on sensitized B lymphocytes themselves can select the target epitopes in the antigen. The BCRs may act as a strict sensor of B lymphocytes to precisely select the target epitopes, even though the number of immunized B lymphocytes is low. [Display omitted] • We have successfully developed optimized B-cell targeting (BCT) technique. • B-cell receptors can work as a strict sensor for selecting target epitopes. • B lymphocytes themselves can select target epitopes through B-cell receptors. • Improved antigen-based hybridoma technology applicable to lower antigenic sites. • Optimized BCT method can generate advanced diagnostic novel monoclonal antibodies. [ABSTRACT FROM AUTHOR]
- Abstract: Copyright of Journal of Immunological Methods is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Abstract:
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