Phytochemical Analysis and Extraction of Lutein using Column Chromatography from Tagetes and Aster plants.

The Asteraceae is one of the widespread family of Angiosperms and is well known for the presence of flavonoids, glycosides and other bioactive constituents. They are reported to have antifungal, antioxidant properties and thus have generated lots of interest in curing and preventing many diseases. In the present study, phytochemical analysis using ethanol, petroleum and chloroform extracts of Aster and Tagetes dried flower samples were analyzed. The phytochemicals such as carbohydrates, alkaloids, glycosides were observed in all extracts of both plant samples except tannins and anthraquinone glycoside chemical tests gave negative results in Aster plant extract. The dried flower sample of both plants were also subjected to column chromatography for separation of Lutein (a fat-soluble xanthophyll). Best results in isolation of Lutein sample were obtained using hexane: ethyl acetate (90:10) mobile phase. [ABSTRACT FROM AUTHOR]

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