Experimental evaluation of different precision criteria applicable to microbiological counting methods.

Publisher: Oxford University Press Country of Publication: England NLM ID: 9215446 Publication Model: Print Cited Medium: Print ISSN: 1060-3271 (Print) Linking ISSN: 10603271 NLM ISO Abbreviation: J AOAC Int Subsets: MEDLINE

Publication: 2020- : Oxford : Oxford University Press
Original Publication: Arlington, VA : AOAC International, c1992-

Colony Count, Microbial* ; Food Microbiology* ; Microbiology*; Algorithms ; Bacteriological Techniques ; Cheese/microbiology ; Eggs/microbiology ; Evaluation Studies as Topic ; Food Contamination ; Laboratories ; Listeria monocytogenes ; Models, Statistical ; Reproducibility of Results ; Sensitivity and Specificity

The dispersion of microbiological counting measurements, when repeating the analysis on the same material both within a laboratory (repeatability) and between laboratories (reproducibility) can be characterized by the organization of interlaboratory studies, where several sets of identical test materials are sent to several laboratories. Using the example of data generated by an interlaboratory study on enumeration of Listeria monocytogenes in foods by the standardized reference method (colony-count technique), 2 types of robust estimators of reproducibility standard deviations, based on the median, were examined, in comparison with the classical estimators, based on the mean. Experimental evaluation indicated that the 3 approaches gave consistent results for most of the combinations. The usual log10 transformation of the enumeration results was also questioned before these calculations were conducted.

Erratum in: J AOAC Int. 2006 Jan-Feb;89(1):11A-13A.

Date Created: 20050709 Date Completed: 20050919 Latest Revision: 20190729

20240829

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