GABAB and CB1 cannabinoid receptor expression identifies two types of septal cholinergic neurons.

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  • Author(s): Nyíri G;Nyíri G; Szabadits E; Cserép C; Mackie K; Shigemoto R; Freund TF
  • Source:
    The European journal of neuroscience [Eur J Neurosci] 2005 Jun; Vol. 21 (11), pp. 3034-42.
  • Publication Type:
    Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Wiley-Blackwell Country of Publication: France NLM ID: 8918110 Publication Model: Print Cited Medium: Print ISSN: 0953-816X (Print) Linking ISSN: 0953816X NLM ISO Abbreviation: Eur J Neurosci Subsets: MEDLINE
    • Publication Information:
      Publication: : Oxford : Wiley-Blackwell
      Original Publication: Oxford, UK : Published on behalf of the European Neuroscience Association by Oxford University Press, c1989-
    • Subject Terms:
      Acetylcholine/*metabolism ; Cholinergic Fibers/*metabolism ; Neurons/*metabolism ; Receptor, Cannabinoid, CB1/*metabolism ; Receptors, GABA-B/*metabolism ; Septal Nuclei/*metabolism; Animals ; Cannabinoid Receptor Modulators/metabolism ; Cell Count ; Cell Size ; Choline O-Acetyltransferase/metabolism ; Cholinergic Fibers/classification ; Hippocampus/metabolism ; Immunohistochemistry ; Male ; Neural Inhibition/physiology ; Neural Pathways/metabolism ; Neurons/classification ; Rats ; Rats, Wistar ; Synaptic Transmission/physiology ; Theta Rhythm ; gamma-Aminobutyric Acid/metabolism
    • Abstract:
      Septohippocampal cholinergic neurons play key roles in learning and memory processes, and in the generation of hippocampal theta rhythm. The range of receptors for endogenous modulators expressed on these neurons is unclear. Here we describe GABA(B) 1a/b receptor (GABA(B)R) and type 1 cannabinoid receptor (CB(1)R) expression in rat septal cholinergic [i.e. choline acetyltransferase (ChAT)-positive] cells. Using double immunofluorescent staining, we found that almost two-thirds of the cholinergic cells in the rat medial septum were GABA(B)R positive, and that these cells had significantly larger somata than did GABA(B)R-negative cholinergic neurons. We detected CB(1)R labelling in somata after axonal protein transport was blocked by colchicine. In these animals about one-third of the cholinergic cells were CB(1)R positive. These cells again had larger somata than CB(1)R-negative cholinergic neurons. The analyses confirmed that the size of GABA(B)R-positive and CB(1)R-positive cholinergic cells were alike, and all CB(1)R-positive cholinergic cells were GABA(B)R positive as well. CB(1)R-positive cells were invariably ChAT positive. All retrogradely labelled septohippocampal cholinergic cells were positive for GABA(B)R and at least half of them also for CB(1)R. These data shed light on the existence of at least two cholinergic cell types in the medial septum: one expresses GABA(B)R and CB(1)R, has large somata and projects to the hippocampus, whereas the other is negative for GABA(B)R and CB(1)R and has smaller somata. The results also suggest that cholinergic transmission in the hippocampus is fine-tuned by endocannabinoid signalling.
    • Grant Information:
      DA00286 United States DA NIDA NIH HHS; DA11322 United States DA NIDA NIH HHS; MH54671 United States MH NIMH NIH HHS; NS30549 United States NS NINDS NIH HHS
    • Accession Number:
      0 (Cannabinoid Receptor Modulators)
      0 (Receptor, Cannabinoid, CB1)
      0 (Receptors, GABA-B)
      56-12-2 (gamma-Aminobutyric Acid)
      EC 2.3.1.6 (Choline O-Acetyltransferase)
      N9YNS0M02X (Acetylcholine)
    • Publication Date:
      Date Created: 20050628 Date Completed: 20050824 Latest Revision: 20131121
    • Publication Date:
      20221213
    • Accession Number:
      10.1111/j.1460-9568.2005.04146.x
    • Accession Number:
      15978014