Analisis Infektivitas Simian-Human Immunodeficiency Virus pada Peripheral Blood Mononuclear Cell Macaca fascicularis dan M. nemestrina Secara In Vitro. (Indonesian)

IN VITRO INFECTIVITY ANALYSIS OF SIMIAN-HUMAN IMMUNODEFICIENCY VIRUS IN PERIPHERAL BLOOD MONONUCLEAR CELL MACACA FASCICULARIS AND M. NEMESTRINA. (English)

Development of human immunodeficiency virus type 1 (HIV-1) vaccines and anti-retroviral treatmentis currently hindered by the lack of models representing prominent symptoms of HIV-1 infections seen in humans. Simian-human immunodeficiency virus (SHIV) was constructed to resolve the limitations of SIVmac model and has been used in nonhuman primate model ofviral infections, particularly infections by the close relatives of HIV-1. Macaca fascicularis and M. nemestrina are being developed as model HIV/AIDS, by using chimeric virus SHIV produced by replacing the nucleotide structure of cyclophilin A binding region, vif gene and nef of HIV-1 with cyclophilin A binding region, vif gene and nef from SIV. The research aims to study the model of HIV/AIDS on nonhuman primates PBMC in vitro using SHIV. In particular, the study aims to obtain information about the capability of SHIV replication in PBMC of M. fascicularis and M. nemestrina. Results showed a cytopathic effect (CPE) in the form of multinucleated giant cells and expression of p24 protein in PHA-stimulated PBMC cultures of M. fascicularis and M. nemestrina after SHIV infection. The conclusion of this study is that SHIV can infect PBMC M. fascicularis and M. nemestrina in vitro based on CPE and expression of p24 protein. [ABSTRACT FROM AUTHOR]

Keterbatasan utama dalam upaya pengembangan vaksin dan pengobatananti-retroviral terhadap infeksi human immunodeficiency virus type-1 (HIV-1) antara lain adalah ketiadaan model hewan yang dapat menunjukkan semua gejala menonjol infeksi HIV-1 seperti pada manusia. Simian-human immunodeficiency virus (SHIV) dikonstruksi untuk mengatasi keterbatasan model SIVmac dan telah dimanfaatkan pada banyak penelitian menggunakan satwa primata untuk mempelajari virus yang memiliki kedekatan dengan HIV-1 pada manusia. Satwa primata Macaca fascicularis dan M. nemestrina digunakan sebagai hewan coba penelitian HIV/AIDS dengan memanfaatkan virus kimera SHIV yang dihasilkan melalui penggantian susunan nukleotida bagian cyclophilin A binding region, gen vif dan gen nefHIV-1 dengan bagian cyclophilin A binding region, gen vif dan gen nef dari SIV. Penelitian bertujuan untuk mengkaji model HIV/AIDS pada PBMC satwa primata secara in vitro menggunakan SHIV. Secara khusus, penelitian ini bertujuan untuk mendapatkan informasi mengenai kemampuan atau daya replikasi dan kinetika SHIV pada kultur sel PBMC asal M. fascicularis dan M. nemestrina secara in vitro. Hasil penelitian menunjukkan gambaran cytopathic effect (CPE) berupa multinucleated giant cell dan ekspresi protein p24 pada kultur PHA-stimulated PBMC M. fascicularis dan M. nemestrina setelah infeksi SHIV. Kesimpulan penelitian ini SHIV dapat menginfeksi PBMC M. fascicularis dan M. nemestrina secara in vitro berdasarkan gambaran CPE dan ekspresi protein p24. [ABSTRACT FROM AUTHOR]

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