Specific identification of Lachesis muta muta snake venom using antibodies against the plasminogen activator enzyme, LV-PA.

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  • Author(s): Felicori LF;Felicori LF; Chávez-Olórtegui C; Sánchez EF
  • Source:
    Toxicon : official journal of the International Society on Toxinology [Toxicon] 2005 May; Vol. 45 (6), pp. 803-6.
  • Publication Type:
    Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Pergamon Press Country of Publication: England NLM ID: 1307333 Publication Model: Print Cited Medium: Print ISSN: 0041-0101 (Print) Linking ISSN: 00410101 NLM ISO Abbreviation: Toxicon Subsets: MEDLINE
    • Publication Information:
      Original Publication: Oxford ; New York : Pergamon Press, c1962-
    • Subject Terms:
      Viperidae*; Antibody Specificity/*immunology ; Crotalid Venoms/*isolation & purification; Animals ; Antibodies/immunology ; Brazil ; Enzyme-Linked Immunosorbent Assay/methods ; Epitopes/immunology ; Mice ; Plasminogen Activators/immunology ; Species Specificity
    • Abstract:
      Sandwich-type enzyme linked immunosorbent assays (ELISA) were developed to detect Lachesis muta muta (bushmaster) snake venom using antibodies against the plasminogen activator enzyme (LV-PA). Antibodies to LV-PA were obtained by immunization of one rabbit with the purified enzyme. The IgG fraction was purified from rabbit blood in a single step on a column of Sepharose-L. m. muta venom and used to coat the microtiter plates. The specificity of the assay was demonstrated by its capacity to correctly discriminate between the circulating antigens in mice that were experimentally inoculated with L. m. muta venom from those in mice inoculated with venoms from Bothrops atrox, B. brazili, B. castelnaudi, Bothriopsis taeniata, B. bilineata, Crotalus durissus ruruima and the antigenic Bothrops (AgB) and Crotalus (AgC) pools venoms used to produce Bothropic and Crotalic antivenoms at Fundacao Ezequiel Dias (FUNED). Measurable absorbance signals were obtained with 1.5 ng of venom per assay. The ELISA was used to follow the kinetic distribution of antigens in experimentally envenomed mice.
    • Accession Number:
      0 (Antibodies)
      0 (Crotalid Venoms)
      0 (Epitopes)
      0 (Lachesis venom)
      EC 3.4.21.- (Plasminogen Activators)
    • Publication Date:
      Date Created: 20050405 Date Completed: 20050609 Latest Revision: 20061115
    • Publication Date:
      20221213
    • Accession Number:
      10.1016/j.toxicon.2004.12.003
    • Accession Number:
      15804530