Evaluation of the EVIGENE VRE detection kit for detection of vanA and vanB genes in vancomycin-resistant enterococci.

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  • Additional Information
    • Source:
      Publisher: Microbiology Society Country of Publication: England NLM ID: 0224131 Publication Model: Print Cited Medium: Print ISSN: 0022-2615 (Print) Linking ISSN: 00222615 NLM ISO Abbreviation: J Med Microbiol Subsets: MEDLINE
    • Publication Information:
      Publication: 2015- : London : Microbiology Society
      Original Publication: 1968-1995: Edinburgh, Livingstone.
    • Subject Terms:
      Vancomycin Resistance*; Bacterial Proteins/*genetics ; Carbon-Oxygen Ligases/*genetics ; Enterococcus/*genetics; DNA, Bacterial/genetics ; DNA, Bacterial/isolation & purification ; Electrophoresis, Agar Gel ; Enterococcus/drug effects ; Enterococcus faecalis/drug effects ; Enterococcus faecalis/genetics ; Enterococcus faecium/drug effects ; Enterococcus faecium/genetics ; Microbial Sensitivity Tests/methods ; Polymerase Chain Reaction/methods
    • Abstract:
      The aim of this study was to evaluate the performance of the EVIGENE VRE Detection kit and compare it with PCR, considered the gold standard for detection of vancomycin-resistant enterococci (VRE). The correlation between the MIC values of vancomycin and teicoplanin using the epsilon test was also determined. In the EVIGENE VRE Detection kit, DNA probes specific for bacterial target DNA sequences are bound to microwell plates. A hundred and ten VRE (104 Enterococcus faecium and six Enterococcus faecalis) and 45 vancomycin-susceptible E. faecium were tested. All VRE strains were found to be positive for the vanA genotype using the EVIGENE VRE Detection kit. All results obtained with the EVIGENE VRE Detection kit were confirmed by PCR. MIC results for the strains also correlated highly with the PCR and kit results. The EVIGENE VRE Detection kit should be used in preference to other methods for detecting resistance genes in all strains, since it is less time-consuming, does not require the handling of hazardous chemicals and has the same specificity as PCR.
    • Accession Number:
      0 (Bacterial Proteins)
      0 (DNA, Bacterial)
      0 (VanA ligase, Bacteria)
      0 (VanB protein, Enterococcus)
      EC 6.1.- (Carbon-Oxygen Ligases)
    • Publication Date:
      Date Created: 20050317 Date Completed: 20050516 Latest Revision: 20200331
    • Publication Date:
      20221213
    • Accession Number:
      10.1099/jmm.0.45789-0
    • Accession Number:
      15770018